PENGARUH PENDEDAHAN CAHAYA PADA POTENSI REGENERASI ANGGREK KELIP (PHALAENOPSIS VIOLACEA H. WITTE) POLIPLOIDI SECARA IN VITRO

• Main Author: Shinta Martha, Madevya
• Format: Final Project
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/55455
• Institution: Institut Teknologi Bandung

Light is one of external factors which promote growth and development in plants. In this study, exposure of different light spectrum was conducted to protocorm-like bodies (PLB) of violet moth orchid (Phalaenopsis violacea) intended to determine the optimum light spectrum needed to promote regeneration competence. Protocorm-like bodies of polyploid P. violacea obtained from colchicine 0.2% (w/v) treatment for 72 hours then PLBs were subcultured 2 times before planted on ½ MS solid medium supplemented with 5% Mas banana extract for 18 weeks. PLBs were exposed under white (positive control), red, and blue LED with photoperiod 18/6 hours light/dark. As negative control, diploid PLBs were exposed to white LED with photoperiod 18/6 hours light/dark. PLBs survival rate (%), total new PLBs, and total shoots were observed. This study found that diploid P. violacea PLBs survival rate were higher (71.29%) than polyploid, but blue light exposure was successful to escalate polyploid survival rate (47.43%) compared to red light (23.71%) and white light exposure (37.86%). Polyploid PLBs have slower growth rate compared to diploid PLB, indicated by total new PLBs and shoots of polyploid P. violacea that were relatively fewer than diploid. On the twelfth weeks, diploid P. violacea formed 10.25 new PLBs, while polyploid P. violacea formed higher number of new PLBs when exposed to blue light (10.5) compared to red light (2.88) and white light (8.38). On the eighteenth weeks, blue light exposure reduced the number of shoots regenerated by polyploid P. violacea (1.00) compared to red light (2.60) and white light exposure (2.60), on the other side diploid P. violacea regenerated 4.40 shoots under white light. Based on the data obtained, this study concluded that blue light was effective to induce polyploid P. violacea PLB formation whereas shoot regeneration was achieved better using white or red light.