LOCALIZED SURFACE PLASMONIC RESONANCE BIOSENSOR WITH SCFV PROTEIN RECEPTOR (SINGLE CHAIN FRAGMENT VARIABLE) FOR COVID-19 DETECTION

• Main Author: Solihin, Aminul
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/55957
• Institution: Institut Teknologi Bandung

Diagnostics plays a very important role in controlling COVID-19 caused by SARSCoV2. This virus has a high rate of spread and has quickly put the world in a pandemic condition. By far the most accurate and gold standard diagnostic method is the PCR-based test. The working principle of PCR is to amplify the genetic material (RNA) of SARS-CoV2. To win against COVID-19, efforts must be made to increase the number of tests. In this study, a localized plasmonic resonance method was used which can be used to detect COVID-19 based on the shift in the resonant frequency wavelength of gold nanoparticles. The receptor in the form of scFv CR3022 recombinant protein is used for elution or purification and concentration to bind COVID-19. The results of the wavelength shift of the resonant frequency towards longer wavelengths were obtained for the detection of positive sampels — RBD (receptor binding domain) SARS-CoV2. In this study, the response of the furthest wavelength shift (4 nm) with bipyramid gold nanoparticles – scFv purification in PBS (phosphate buffered saline) and bikarbonat was obtained. While the non-specific sampel test – IB (infectious bronchitis) – found a wavelength shift towards a shorter wavelength with the furthest shift (-3.1 nm) detected with gold sphere nanoparticles – sFfv concentration in Tris Cl. In the test sampel mixture of RBD and IB detected with gold nanoparticles sphere – scFv concentration obtained a shift response of 0.4 nm. These results confirm that scFv binding is specific for SARS-CoV2.