FORMATION OF BIOFILM IN SUPPORTING MEDIA POLYETHYLENE TEREPHTHALATE (PET) FIXED-BED ANOXIC AND AEROB SYSTEM USING BATCH REACTOR

• Main Author: Fauzi, Mhd.
• Format: Theses
• Language: Indonesia
• Subjects: Teknik saniter dan perkotaan; teknik perlindungan lingkungan
• Online Access: https://digilib.itb.ac.id/gdl/view/56010
• Institution: Institut Teknologi Bandung

Research on the use of supporting media such as PET, HDPE and Acrylic in attached reactors has been carried out to obtain a specific surface area, so that large numbers of microorganisms can be attached. The use of PET plastic bottles as a supporting media raises a new concern, namely the presence of secondary microplastics which are thought to have come from supporting media during the processing. This research was conducted on fixed-bed reactor (FBR) anoxic and aerobic batch system using artificial domestic wastewater. The artificial wastewater used has a composition of C6H12O6, NH4Cl and KH2PO4 as a source of carbon, nitrogen and phosphorus. This study consisted of two experiments. The first experiment was to see the formation of biofilms by calculating the weight gain of total attached solids (TAS) using the gravimetric method, thickness and surface of the biofilm using a microscope and scanning electron microscope (SEM) on 10th, 20th, 30th, 40th, 50th, 60th, 70th and 80th day (anoxic) and 5th, 10th, 15th, 20th, 25th, 30th, 35th and 40th day (aerobic). Three types of supporting media used in the first experiment were PET, HDPE and Acrylic. The second experiment was to see the removal of organic compounds and nutrients, the kinetics of microorganism growth and removal of substrates and the presence of PET supporting media as microplastics. Based on this study the best media for the growth of microorganisms is PET with TAS weight and biofilm thickness formed is 0.90 mg/cm2 and 347.37 ± 7.11 ?m in anoxic reactor on day 80, while in aerobic reactor 1.33 mg/cm2 and 428.83 ± 3.10 ?m on day 40. Artificial wastewater treatment in FBR anoxic and aerob used PET supporting media with COD variety 200, 300, 400, 500, 600 mg/L can removal of organic and nutrien. The results of removal of organic compounds and nutrients using PET as a support media in the anoxic reactor were COD 91.73 ± 2.84%, ammonia 75.59 ± 0.89%, nitrite 74.27 ± 0.42%, nitrate 84.02 ± 0.82%, TN 70.20±0,52% and TP 77.37 ± 0.76%, with the selected substrate removal kinetics is the Singh method which produces a kinetics value (k1) of 0.4626 ± 0.03 / hour. Whereas in the aerobic reactor the efficiency of COD removal was 85.76 ± 0.59%, ammonia 76.59 ± 0.83%, nitrite 76.09 ± 0.66%, nitrate 64.30 ± 0.42%, TN 77.20±0,94% and phosphate 86.54 ± 0.68%, with the selected substrate removal kinetics is the Singh method which produces a kinetics value (k1) of 1.60 ± 0.05/hour. The kinetics value of the growth of microorganisms produced in the anoxic reactor were µmax 1.87/day and Ks 80.28 mg / L, while in the aerobic reactor the values were µmax 2.72/day and Ks 78.72 mg/L. The presence of PET microplastics detected in the two reactors did not come from the supporting media, but from the sludge used in the anoxic seeding 75 days and aerobic seeding 40 days . This is evidenced by the calculation of the microplastic mass balance in the reactor and identification using FTIR.