ELECTROCHEMICAL BIOSENSOR BASED METAL ORGANIC FRAMEWORK CU-NH2BDC FOR HEPATITIS B SURFACE ANTIGEN DETECTION

• Main Author: Rezki, Muhammad
• Format: Theses
• Language: Indonesia
• Subjects: Teknik (Rekayasa, enjinering dan kegiatan berkaitan)
• Online Access: https://digilib.itb.ac.id/gdl/view/56218
• Institution: Institut Teknologi Bandung

Metal-organic framework (MOF) materials offer a wide range of promising applications due to their advantageous properties, including open micro- and mesopores and flexible functionalization. Herein, a facile synthesis via a solvotermal method was successfully employed to prepare Cu based MOF (Cu-NH2BDC). TEA and PVP play an important role to govern the growth of the obtained MOF structures. The Cu-NH2BDC was incorporated with the amine group hence improve its functionalization to immobilize bioreceptor towards the detection of Hepatitis B infection biomarker, i.e., Hepatitis B surface antigen (HBsAg). The immobilization of the antibody on the electrode surface is anticipated through a covalent interaction between the carboxyl group of antibodies and the amino-functional ligand in Cu-MOF via EDC/NHS coupling. The Cu-NH2BDC MOF not only act as a nanocarrier for the antibody immobilization but also acts as an electroactive material to generate the electrochemical signal. The electrochemical sensing performance was characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and differential pulse voltammetry (DPV). The obtained current response proportionally decreases with the increase of HBsAg concentration. Thus, quantitively HBsAg detection was successfully achieved. This strategy successfully resulting in a wide linear range detection of HBsAg from 1 ng/ml to 500 ng/ml with a limit of detection (LOD) of 0.73 ng/ml as well as good selectivity and reproducibility. The current approach provides a facile and low-cost synthesis process and paves the way to utilize MOF-based nanomaterials for electrochemical biosensor.