PERKEMBANGAN PROSES PRODUKSI ARTEMISININ SEMISINTESIS MENGGUNAKAN MIKROBA

• Main Author: Adiwijaya, Royyan
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/56409
• Institution: Institut Teknologi Bandung

Malaria is a very deadly infectious disease. According to 2019 research, the severity in tropical countries like Indonesia is 100 times of subtropical regions. In addition, there is no vaccine for malaria so far, so curative treatment is still being relied upon. Conventional curative treatment has many obstacles such as high side effects (kinin), high resistance rate (chloroquine), and limited spectrum (all therapies except artemisinin). Artemisinin is currently the best curative option, but limited supply makes artemisinin relatively expensive compared to others. Semisynthetic production using microbes is considered the most extensible to an industrial scale. The purpose of writing this paper is to find the development, critical step, and the best host and bioreaction conditions to increase the production of artemisinin by microbial engineering. The data sources used are international journals (PubMed and Google Scholar) and case reports (WHO, Riskesdas, etc.). Source eligibility criterias are sources discussing artemisinin production, especially microbial engineering methods as well as other data that can support it. The study methodology was carried out with a comparative causal approach. The conclusion from this study is that the semisynthetic method that's developed from single gene transformation to the application of coexpression of multiple genes has a critical step in the identification and manipulation of the rate-determining step in the artemisinin bioreaction. The best microbial host for the production of artemisinin semisynthesis is S. cerevisiae with ideal conditions are 30°C, pH 5-6, inoculum level of 2.5-14%, using glucose-ethanol carbon and organic nitrogen sources in a fed-batch bioreactor.