EUGENYL ACETATE PRODUCTION FROM CLOVE OIL WITH IMMOBILIZED LIPASE ENZYME

• Main Author: Zaidan Amir, Muhammad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/56493
• Institution: Institut Teknologi Bandung

Cloves have a variety of benefits that have been widely felt. One of the derivative component of cloves, namely eugenyl acetate has a very large utilization potential. Commercially eugenyl acetate has been widely produced with acidic or basic catalysts. However, in practice there are some weaknesses that are corrosive to the equipment and also the treatment of waste reaction. Therefore, this research was conducted in order to develop enzymatic production of eugenyl acetate. The enzyme used was Candida rugosa lipase which was immobilized by the adsorption on zeolites support. The concentration of immobilized enzyme adsorbed on the catalyst was 7.19 mg / ml or 71.9% and the optimum activity of immobilized enzymes was achieved at a temperature of 45oC and pH 7. In this study, the reaction products were analyzed by GC-MS and the chromatogram showed 4 peaks, namely eugenol, eugenyl acetate, acetic acid, and ethanol. The production of eugenyl acetate was carried out through the esterification reaction of clove oil for 2 hours with a molar ratio of eugenol to acetic anhydride 1: 3, temperature 50°C, enzyme concentration 5%-eugenol weight, and a shaking speed of 150 rpm. From literature also found that enzyme kinetics model for eugenol esterification still has 2 possibilites, which is ping pong bi bi and sequential bi bi reactions. For ping pong bi bi, it was found that from the Km values, lipase enzyme has higher affinity toward acetic anhydride rather than eugenol. Also, it is evident that an increase in reaction temperature will result in an increase in esterification rate (Vmax).