ANTIMALARIAL STUDY OF NANOPARTICLE CHITOSAN, POLOXAMER, AND PLGA BASED CONTAINING ANTISENSE OLIGONUCLEOTIDES OF EBA-175 AND DHODH

• Main Author: Maulidia Rahayyu, Annisa
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/56667
• Institution: Institut Teknologi Bandung

Malaria is one of the infectious disease caused by Plasmodium falciparum. The resistent in malaria treatment is still a problem in elimination malaria cases globally. A new antimalarial drug, nanoparticles (NP) containing antisense oligonucleotides (ASO) specific to eba-175 and dhodh targeted were developed. This study aims to evaluate the effect of ASOs NP on growth inhibitory activity and decreased of eba-175 and dhod genes expression in P. falciparum. The formula of nanoparticle chitosan, poloxamer, and PLGA based prepared containing ASO by nanoprecipitation method were produce the particle size characteristic <200 nm, polydispersion index <0.5 and positive zeta potential. The inhibitory activity by microscopic method showed significant effect (P<0.05) when compared to negatives control. % Inhibiton reaching >80% at any ASOs NP concentration (0.0125 µM; 0.025 µM; 0.05 µM; dan 0.1 µM). Ratio from relative quantification (R) of eba-175 gen gave lower expression by 1.27 times; 1.14 times; and 1.29 times at concentration of 0.025 µM; 0.05 µM; and 0.1 µM (respectively). Furthermore, in NP dhodh targeted was seen gave expression 2.15 times lower than negative control at 0.05 µM and 1.35 times lower at 0.1 µM. However the decrease in expression gen was not significantly different (P>0.05). The presence of P. falciparum growth inhibition was also shown in NP blank. These problem need to be further confirmed.