ISOLATION OF THE ACTIVE COMPOUNDS OF TYROSINASE ENZYME’S INHIBITOR FROM METHANOL EXTRACT OF MENIRAN HERBS (PHYLLANTHUS NIRURI LINN.)

• Main Author: Salsabila, Syefira
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/56679
• Institution: Institut Teknologi Bandung

Meniran (Phyllanthus niruri L.) is a wild plant in the Euphorbiaceae family, lives in tropical climates which has antioxidant activity. It has a high phenolic content. Plants with high phenolic content and high antioxidant activity can also produce high inhibition of tyrosinase activity. The purpose of this study was to determine the inhibitory activity of the tyrosinase enzyme by methanol extract of meniran (P. niruri L.) and to isolate secondary metabolites from the extract and fraction of meniran (P. niruri L.) which actively inhibits the tyrosinase enzyme. Tyrosinase inhibition activity used the 96-well microplate method in vitro with kojic acid as positive control. Separation dan purification using chromatography techniques. Methanol extract and n-hexane fraction had tyrosinase enzyme inhibitory activity with IC50 of 11,05±0,16 and 2,57±0,08 mg/ml, respectively. Three bioactive compounds have been isolated. Based on UV spectrum and HPLC overlay with standard, isolate 1 is phyllanthin, and based on UV, 1H-NMR and 13C-NMR spectra, isolate 3 is hypophyllanthin. Isolate 2 based on UV spectrum is considered as compounds from the lignan group. Phyllanthin showed inhibitory activity of enzyme tyrosinase with IC50 264,57±3,74 ?g/ml. Meniran extract and phyllanthin were recently reported to have inhibitory acitivity against the tyrosinase enzyme