DEVELOPMENT OF METABOLITE AND GENETIC MARKER ON JAVA TEA PLANT (ORTHOSIPHON ARISTATUS) AS A NUTRACEUTRICAL RAW MATERIAL

• Main Author: Kasih, Mentari
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/57070
• Institution: Institut Teknologi Bandung

Orthosiphon aristatus (Java Tea) is one of the common medicinal plants found in Indonesia and cultivated by various Indonesian ethnic groups. Various studies have shown that cat whiskers have various active compounds useful in pharmacological activity, such as sinensetin which is the identity compound for simplicia, and rosmarinic acid, which is the main antioxidant compound of cat whiskers. This research aims to develop a metabolite marker and genomics marker on Java Tea. Dry leaf samples of cat whiskers from 10 ethnicities in Indonesia, where each ethnic group has five-leaf samples from five different individuals. Samples were obtained from the Center for Research and Development of Medicinal Plants and Traditional Medicines (B2P2TOOT). The development of metabolite markers was carried out using HPLC (High-Performance Liquid Chromatography) on sinensetin and rosmarinic acid, where the chromatogram data were processed using MS. Excel and statistical tests using One Way Anova and Kruskal Wallis and further testing using Turkey HSD with the IBM SPSS Statistics 25 program. Development of genomic markers was carried out on white cat whiskers obtained from the Parongpong area, West Bandung Regency. DNA isolation of the purple java tea genome using modified CTAB method and genomic DNA sequencing using Oxford Nanopore technology. Based on the results of the metabolite profile of sinensetin and rosmarinic acid, there is a significant difference (p-value <0.05) in the concentration of rosmarinic acid in cat whiskers at an altitude of 100-1000 m asl, namely the Majapahit, Kalisusu, Kutai and Peminggir ethnicities compared to other ethnicities where indicates a higher concentration value. Sinensetin metabolites did not show a significant difference (p-value> 0.05). The results of DNA sequencing readings obtained were as many as 249 reads sequences with a range of base length for each sequence 238-30,817 bp, the average base length was 4,140.1 bp and the total length of the reads obtained was 1,031,664 bp. After that, the raw data from the sequencing readings was used for SSR characterization where obtained 351 perfect SSRs, 1,232 imperfect SSRs and 36 compound SSRs using Krait v software. 1.3.3. The primer design was produced as many as 329 pairs of primers. These primers will later be used to develop SSR markers on Java Tea, where these genomic markers can later be applied to cat whiskers plant populations to map the genetic diversity of Java Tea in Indonesia and develop varieties that have high levels of rosmarinic acid and sinensetin.