EXTRACELLULAR DNA OF FUSARIUM OXYSPORUM F.SP. CUBENSE AS AN ELICITOR TO ENHANCE RESISTANCE OF BANANA PLANTLET

• Main Author: Hamida Hanisia, Ristag
• Format: Theses
• Language: Indonesia
• Subjects: Teknologi
• Online Access: https://digilib.itb.ac.id/gdl/view/57075
• Institution: Institut Teknologi Bandung

Fusarium wilt is one of the major causes of global losses in the banana industry. The application of extracellular DNA (eDNA) is expected to be a promising technology as a natural pesticide for this disease. eDNA is a fragment of genomic DNA or organelle that is outside the cell and grouped into two groups based on the cell's perception, namely self and non-self. eDNA has potential in agriculture, namely as pest control (self eDNA) and vaccine for plants (non-self eDNA). This study aimed to evaluate whether the eDNA of Fusarium oxysporum f.sp. Cubense (Foc) can inhibit growth for Foc itself (self-inhibition test) and increase resistance in banana plants (elicitation test). In the self-inhibition test, Foc TR4 spores were inoculated on PDA medium treated with fragmented Foc eDNA at concentrations of 80, 400, and 800 ?g/mL. Then calculated the growth of the colonies formed. The results showed that the administration of eDNA with concentrations ranging from 400 ?g/mL could significantly inhibit the growth of Foc TR4 spore germination. While elicitation was carried out on banana plantlets in the interaction medium which was given eDNA Foc suspension with final concentrations of 40, 80, and 200 ?g/mL with deion as a negative control and 5 uM salicylic acid as a comparison of elicitor chemical compounds. Furthermore, banana cultures that had been elicited for 1 day were treated with pathogen infection with the addition of a spore suspension with a concentration of 106 spore cells/mL. Administration of eDNA with a concentration of 200 ?g/mL could increase the resistance of Cavendish bananas to Foc infection from susceptible to tolerant with a leaf symptom index of 1.6 and the rhizome discoloration index of 2.6. In addition, the accumulation of ROS, O2- and H2O2 compounds was observed on H-1 after elicitation and a significant increase in the relative expression of CAT, PR1, and PR3 genes on H-9 post-infection. Based on this, the application of elicitation of eDNA Foc TR 4 with a concentration of 200 ?g/mL can be considered in the development of eDNA as an environmentally friendly pesticide to increase resistance and quality of banana plants.