STUDY ON PROPERTIES AND PROCESSING OF ALGINATE-BASED BIOINK FOR ORGANOID 3D BIOPRINTING

• Main Author: Leonardo, Michael
• Format: Final Project
• Language: Indonesia
• Subjects: Bahan bangunan
• Online Access: https://digilib.itb.ac.id/gdl/view/57279
• Institution: Institut Teknologi Bandung

Organoid is a type of 3D cell-culture to model biological systems that can be fabricated through 3D bioprinting. The hydrogel scaffold fabricated via 3D bioprinting is printed using bioink, consisted of polymeric materials and living cells. Alginate has the potential to be developed as bioink, but studies are still needed to obtain optimum alginate-based bioink. In addition, there is still a few studies regarding guideline on the organoid bioprinting process using alginate-based bioink. Hence, a study of alginate and its potential as a bioink was carried out in terms of rheological parameters, printability, physical and mechanical properties, and biocompatibility; as well as a guideline for organoid bioprinting process through literature study method. As bioink, alginate can be optimized through gelatin addition. The result of studies shows that alginate-gelatin mixture can improve the properties of each constituent and has the potential as bioink in organoids bioprinting. A review of parameters indicates that the optimum alginate-based bioink should have viscosity range of 0.4-3 Pa.s; density around 1.05 g.cm-3; shear stress during extrusion lesser than 100 Pa; and use of CaSO4 as crosslinker and DMEM solution in bioink preparation. Gelatin addition increases bioink’s rheology and mechanical properties, excessive addition leads to decrease in printability, and influence the biocompatibility. Organoid bioprinting process should go through organoid determination; scaffold and bioink design; configuration of printing parameters; printing process; evaluation of design, properties, and performance.