SECONDARY METABOLITES FROM SEED SANDORICUM KOETJAPE AND BIOACTIVITY TEST

• Main Author: Ratna Putri, Erien
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/57290
• Institution: Institut Teknologi Bandung

Indonesia is an agricultural country that has various types of plants with various benefits and uses. One of these type of plants is Sandoricum koetjape. It has been reported that this plant contains secondary metabolite groups, such as terpenoids, flavonoids, limonoids, saponins, tannins, and glycosides. The content of these secondary metabolites shows a variety of bioactivity, namely as an insecticide, antifeedant, insect repellent, anti-inflammatory, antioxidant, cytotoxic, and anti- tumor. This study explores secondary metabolites in the S. koetjape plant that grows in the province of Lampung. As far as the authors are concerned, this species has never been studied or reported. This research was conducted by isolating secondary metabolites from S. koetjape seed extract through several stages, namely extraction, fractionation, and purification using various chromatography methods. The molecular structure of the isolated compound was determined based on spectroscopic data (1D- NMR and 2D-NMR). Then the new compounds obtained were evaluated as tyrosine kinase inhibitors (EGFR, HER2, HER4, IGFR, InsR, KDR, PDGFR?, and PDGFR?). In this study, four pure compounds were obtained, namely cipaferen G (41), 3-epi- cipadonoid C (42), koetjapin B (21) and one new compound in the cipadesin type limonoid class (43). Based on the results of the bioactivity test on 8 receptor tyrosine kinases, it was found that the inhibition of cipaferen G (41) was 83%, 3-epi-cipadonoid C (42) was 84%, and koetjapin B (21) was 83%, meaning that it has weak activity against PDGFR-b. Meanwhile, 3-epi-cipadonoid C (42) had moderate activity against InsR (67%), and cipadesin-type limonoid compound (43) had weak activity against HER2 (85%).