PERFORMANCE EVALUATION OF XYLITOL PRODUCTION FROM OIL PALM EMPTY FRUIT BUNCH (EFB) HYDROLISATE IN REPEATED BATCH REACTOR

• Main Author: Adam, Abdurrahman
• Format: Theses
• Language: Indonesia
• Subjects: Teknik kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/58179
• Institution: Institut Teknologi Bandung

Indonesia in 2021 has been predicted to produce up to 2.2 million tons of Oil Palm Empty Fruit Bunch (OPEFB). Bioprocess pathway to produce xylitol whilst utilizing the OPEFB has been reported as a more eco-friendly and sustainable alternative way. Although, xylitol industries are still rarely using this method due to under performance and there is still lack of successful evidence in higher scale of fermentation. Previous study has reported that engineering the reactor configuration could increase the performance of fermentation when using D. hansenii ITB CCR85 in laboratory Erlenmeyer flask scale. Therefore, this research performed xylitol fermentation in 2-L volume bioreactor and apply the novel repeated batch fermentation to evaluate the xylitol fermentation performance using OPEFB hydrolysate. Also, the research was done using synthetic media to observed the effect of non-detoxified hydrolysate media to the xylitol production’s performance. Repeated batch configuration can increase between 71-1800% yield and 67-900% productivity of xylitol fermentation. Compared to synthetic media, D. hansenii’s performance was not lowered using undetoxified OPEFB hydrolysate media. The best xylitol fermentation performance of D. hansenii ITB CCR85 is 29% g xylitol / g xylose yield in synthetic media. Whereas, using the hydrolysate media, the best performance only resulted in 12% g xylitol /g xylose yield and 0.002 g/L/h productivity. Unfortunately, the fermentation process still is under-optimized due to low xylose concentration, low xylose to glucose ration, and low initial cell concentration. It was also found that the range of optimal DO concentration in this research was between 0-0.2 mg/L O2. This research has given a clearer picture on the behaviour of D. hansenii ITB CCR85 strain when converting OPEFB hydrolysate-based xylose into xylitol in 2-L scale bioreactor.