NUCLEOTIDE SEQUENCE OF COII/TRNALYS INTERGENIC REGION IN MITOCHONDRIAL DNA FROM DIFFERENT SAMPLE IN AN INDIVIDUAL

• Main Author: Mira Supena, Christina
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/5895
• Institution: Institut Teknologi Bandung

</i><b>Abstract:</b><i><br /><br /> The control region of human mitochondrial DNA (mtDNA), the displacement loop (D-Loop), is the most polymorphic region that are concentrated in the Hypervariable Segment 1 (HVS1) and Hypervariable Segment 2 (HVS2). Polymorphisms in that region commonly used to study human evolution, ethnic relationship, and migration of population. The COII/tRNALys intergenic region is one of the most studied mtDNA polymorphisms, which can show 9-bp deletion because of lost tandem sequence CCCCCTCTA. The aim of this study was to know whether nucleotide sequence of COII/tRNALys intergenic region in different tissue from an individual has the same mutation. This study used different sample from an individual, there are hair, blood, and ephitel of mouth. The sequences of 0.4 kb PCR fragment from mtDNA confirmed COII/tRNALys intergenic region. Electrophoregrams showed no different sequence in all samples and each sample has nucleotide sequence approximately 390 bp. Analysis of nucleotide sequence with standard CRS (Cambridge Reference Sequence) showed deletion 9-bp mutation in COII/tRNALys intergenic region. The result make sure that individual identification can be investigated with only one of those samples as long as related to 9-bp deletion.