CONVERSION OF CORN EXCTRACT INTO BIOETHANOL
ABSTRACT: <br /> <br /> <br /> <br /> The main issue of fuels availability in the world is the limited amount of raw material from fossils. Bioethanol is a potential alternative to replace fuel, it is ethanol produced from microorganism assisted biomass fermentation. Polysa...
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Format: | Final Project |
Language: | Indonesia |
Online Access: | https://digilib.itb.ac.id/gdl/view/5941 |
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Institution: | Institut Teknologi Bandung |
Language: | Indonesia |
Summary: | ABSTRACT: <br />
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The main issue of fuels availability in the world is the limited amount of raw material from fossils. Bioethanol is a potential alternative to replace fuel, it is ethanol produced from microorganism assisted biomass fermentation. Polysaccharide, in form of cellulose or amylum, is the raw material used to produce bioethanol. Producing bio ethanol from corn exctract using amylase and glucoamilase from sweet corn was performed in this research. To produce glucose, corn extract was hydrolysed with assistance of alpha-amylase. Assisted by Saccharomyces cerevisiae, glucose then fermented to produce bioethanol. The objective of the research was to determine the optimum condition for the hydrolysis process of corn extract which assisted by alpha-amylase, then to perform glucose fermentation at the optimum condition. The optimum concentration for the hydrolysis of corn extract, using 0.3 g/mL of alpha-amylases concentration, was 0.9 g/mL and the optimum incubation was 90 minutes at 35 oC temperature. The fermentation of glucose which produce by corn exctract hydrolysis assisted by Saccharomyces cerevisiae at optimum condition, produced 13,591 % v/v of bioethanol. Means each 1 mmol of sweet corn extract can be converted into 2,47 % v/v of bioethanol at optimum hydrolysis condition. |
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