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Abstract : <br /> <br /> <br /> <br /> Genetic polymorphism found in genes expressing drug-metabolizing enzymes is one factor affecting drug pharmacokinetics. CYP2C19 is one of the gene encoding cytochrome P-450 enzymes with a high genetic polymorphism frequency in Asia. T...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/5992 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Abstract : <br />
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Genetic polymorphism found in genes expressing drug-metabolizing enzymes is one factor affecting drug pharmacokinetics. CYP2C19 is one of the gene encoding cytochrome P-450 enzymes with a high genetic polymorphism frequency in Asia. This study has determined the allel frequencies of CYP2C19*2 and CYP2C19*3. DNA was isolated from 30 random blood samples. Allele identification was performed by Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method. The amplified DNA fragments before and after restriction enzyme digestion with SmaI (CYP2C19*2) and BamHI (CYP2C19*3) were analyzed using polyacrylamide gel electrophoresis and visualized by silver staining. Chi-squared test was used to determine the Hardy-Weinberg equilibrium in the studied sample population. PCR-RFLP results showed individuals with homozygous wild-type CYP2C19*1/*1 (n=17), heterozygous CYP2C19*1/*2 (n=11), and heterozygous CYP2C19*1/*3 (n=2). Neither homozygote CYP2C19*2/*2 and CYP2C19*3/*3 was found nor heterozygote CYP2C19*2/*3. Allele frequencies of CYP2C19*2 and CYP2C19*3 were 18.3% and 3.3%, respectively, with a prevalence of poor metabolizer (PM) of 21.7% and the population sampled in this study was in Hardy- Weinbergs equilibrium. |
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