PEMODELAN PRODUKSI LIPID DARI MIKROALGA HAEMATOCOCCUS PLUVIALIS YANG DIKULTIVASI MENGGUNAKAN MEDIA PUPUK KOMERSIAL

• Main Author: Rahmawati, Anasya
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/61014
• Institution: Institut Teknologi Bandung

Lipids are one of the main constituents in microalgae, which have many functions in bioindustry. Haematococcus pluvialis one of species microalgae with high lipid content 20-37% (w/w). One of the factors that affect the lipid productivity of microalgae is the nutrient content in media cultivation. Alternative media, one of which is commercial fertilizer, is a type of media that generally contains the main nutrients for microalgae growth but different compositions and amounts from standard media. Currently, this type of media has been developed for cost efficiency of microalgae cultivation. Haematococcus pluvialis has been successfully cultivated in several alternative media, such as NPK fertilizer and NPK+M. However, the mechanism of nutrient content in alternative media can affect the lipid content of Haematococcus pluvialis has not been explained. The aims of this study were to determine the productivity of Haematococcus pluvialis cultivated on fertilizer media, to model lipid accumulation based on nitrogen quota, and determine the effect of nutrient content in media on lipid accumulation of Haematococcus pluvialis cultivated on fertilizer media based on secondary data modeling. The research begins with simple research and continues with secondary data modeling. In a simple research, Haematococcus pluvialis was cultivated in stages on 1L and 5L scale using commercial fertilizer media. Secondary data modeling was carried out using growth data of Haematococcus pluvialis cultivated on several alternative media such as WC, NPK, and NPK+M using a lipid accumulation model based on nitrogen quota. The results showed that the productivity of Haematococcus pluvialis cultivated on Nutricomp-D commercial fertilizer through a simple study was 11.25 mg/L.day. Models of nitrogen uptake and lipid accumulation based on nitrogen quota and shift of protein to lipid biosynthesis when nitrogen-deficient media are not suitable for modeling nitrate uptake and lipid accumulation in Haematococcus pluvialis cultivated on alternative media. Haematococcus pluvialis cultivated on alternative media with lower initial nitrate content resulted in higher lipid accumulation, with a model showing that lipid productivity increased from 0.46 mg/L.day to 1.52 mg/L.day.