MODELLING AND SIMULATION OF L-SERINE FERMENTATION IN BATCH BIOREACTOR

• Main Author: Arib Muhsin, Fakhri
• Format: Final Project
• Language: Indonesia
• Subjects: Teknologi
• Online Access: https://digilib.itb.ac.id/gdl/view/61175
• Institution: Institut Teknologi Bandung

L-Serine is a conditional non-essential amino acid that has many uses in pharmaceutical, food, and cosmetics industries. L-serine can be produced by extraction, chemical synthesis, fermentation, and enzymatic conversion. One of the methods that has seen extensive development in recent years is fermentation, because of its applicability in existing industries. To achieve higher product yield, many genetic modifications have been tried on some biocatalysts. As of this research, there has not been any research done to model and simulate recombinant microorganisms for industrial production design. In this study, modeling and simulation were done for some recombinant microorganisms (biocatalysts), and a bioreactor for industrial production of L-serine was designed. Modeling was started by extracting data to obtain parameters of some kinetic models. The kinetic model tested in this study are Monod growth model, modified Monod model with product inhibition, and modified Monod model with thermodynamic considerations. The kinetic parameters from recombinant biocatalysts with highest product to substrate yield was used to simulate product and biomass formation with various initial substrate and biomass concentrations. Bioreactor design was done for a production capacity of 120 kg L-serine/day with batch operation. The result of this study showed that fermentation kinetics for most recombinant biocatalysts tested were fitted best with Monod model. Modified biocatalyst with highest yield was Corynebacterium glutamicum S7(S6-pgi-pfkA-gapA), with YP/S of 0.30 g-Lserine/ g-glucose. Simulation results showed that as the concentration of initial substrate and biomass concentration increases, the rate of biomass production also increases, while the time needed for fermentation decreases. At initial substrate concentration of 100 g L- 1, the maximum titer of fermentation is 29.36 g L-1 , which was used for bioreactor design basis. The bioreactor design result has bare module cost of US$395,661 with operating expenses (raw materials, utility system, and labor cost) of US$196,693/year and sales of US$2,028,000/year.