KULTUR JARINGAN DAN STUDI LITERATUR TRANSFORMASI TRANSIEN AGROBACTERIUM TUMEFACIENS KE SEL NICOTIANA TABACUM

• Main Author: Restu Dewati, Mumpuningtyas
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/61670
• Institution: Institut Teknologi Bandung

Choleostatic liver disease results from obstruction or reduction in the flow of bile that is secreted into the duodenum. Ursodeoxycholic acid (UDCA) is the first line of treatment for choleostatic liver diseases, such as primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). UDCA is a steroid compound that is produced in the liver and acts as a bile acid to help dissolve gallstones. The low production of UDCA in the human liver is a challenge to find a method of producing this drug, one of which uses transformation through microorganism. Transformation with Agrobacterium tumefaciens is a method of producing protein or medicinal compounds that is often used for transgenic plants because of its safety and fast gene expression. Prior to the transformation, plant tissue culture of N. tabacum was performed. From the results of tissue culture, it was successful to grow N. tabacum into whole plants without any contamination. This culture will be used for genetic transformation of the genes that responsible in UDCA biosynthesis. Literature review of genetic transformation that mediated by A. tumefaciens was conducted through two sources Google Scholar and Pudmed to find factors that influencing the efficiency of transformation. Detection of the success of the transformation was carried out through the GUS histochemical test as well as through the PCR test. It was found that the optical density of the cultured bacteria, the addition of fenolic compounds and surfactant, and the duration of co- cultivation affected the success of the transformation.