AKTIVITAS ANTIDEPRESAN KONSENTRAT JUS BUAH PARE (MOMORDICA CHARANTIA L.) PADA MENCIT DDY

• Main Author: Tyas Pangestu, Sandi
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/61916
• Institution: Institut Teknologi Bandung

Depression is a mental disorder that is characterized by excessive sadness, loss of interest, loss of pride, excessive guilt, low self-worth, sleep disturbances, indigestion, fatigue, and difficulty concentrating. Various pharmacological therapies to treat depression have been developed and applied, but the use of antidepressant drugs still has drawbacks, such as dangerous drug side effects. This study was conducted to investigate the antidepressant activity of bitter melon juice concentrate (Momordica charantia L.) by observing the immobility time in the forced swim test method, measuring the concentrations of the neurotransmitters serotonin, dopamine, norepinephrine, as well as cortisol in the brain. Antidepressant activity test was carried out on ddY mice which were divided into five groups, the reference group receiving 2,6 mg/kg bw fluoxetine HCl, the vehicle group receiving 100 mg/kg bw CMC-sodium, the test groups, each receiving 50, 100, 200 mg/kg bw of bitter melon extract. The immobility time was measured for 6 minutes on the 7th and 14th days. The levels of neurotransmitter and cortisol were measured using ELISA method. The results showed that the juice concentrate of bitter melon at doses of 100 and 200 mg/kg bw reduced immobility time in FST testing (p<0,05). Bitter melon juice concentrate at 50 mg/kg bw increased the concentration of serotonin (p>0.05), dopamine (p<0.05), and decreased the hormone cortisol level (p<0.05). Bitter melon juice concentrate at 100 mg/kg bw increased the concentration of serotonin, dopamine, and norepinephrine, although was not significant compared to the vehicle group (p>0.05). Bitter melon juice concentrate at 200 mg/kg bw increased the concentration of norepinephrine but not significant statistically (p>0.05), and reduced the concentration of the hormone cortisol (p<0.05). Overall, the results of this study indicate an improvement in the parameters of the concentration of neurotransmitters and the cortisol concentrations after administration of bitter melon juice concentrate. Thus, bitter melon juice concentrate has the potential as antidepressant activity.