#TITLE_ALTERNATIVE#

• Main Author: 23003018, Haryono<br>NIM.
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/6199
• Institution: Institut Teknologi Bandung

<b></i>Abstract :</b><i><p align="justify"><br />Biological wastewater treatments usually produce activated sludge residue in a large amount. Therefore, the sludge handling management in a wastewater treatment is one activity that takes the most relative financial allocation. Microorganisms in the form of bioagregate are the main component of activated sludge. It generally has an ability to produce EPS (extracellular polymeric substances). The important components in EPS are polysaccharides (EPS_s) and proteins (EPS_p). The activated sludge contains more than 80% of EPS. EPS recovery from the activated sludge may be done by many extraction methods. The selected extraction method will determine production of EPS and its composition.<br /><p align="justify"><br />EPS has extensive roles in the practical activity, such as in medical sectors, food industries, pharmacy industries, petroleum exploitations and wastewater treatments. Especially in the wastewater treatment activity, EPS has been used as an adsorbent of heavy metal pollutants.<br /><p align="justify"><br />Six of the extraction methods to extract EPS from the activated sludge have been studied in this research, such are: Middle Speed Centrifugation, Regular Centrifugation, Sodium Hydroxide Extraction, EDTA Extraction, Steaming Extraction and Sonication. The activated sludge used in this research was the sludge residue from wastewater treatment of a textile industry. The best extraction method was determined based on the EPS production (mg EPS/g TSS) through measurement consentration of polysaccharides and proteins in EPS solution. EPS solution in the following step would be tested its performances as adsorbent of copper heavy metal through suitable isotherm adsorption equation and average maximum adsorption capacity. Adsorption process was applied to 175 ml of Cu solution that varied in concentration 10, 20, 50, 80 and 100 mg/L in 280 ml of SPE solution which contained 37.5 mg EPS.<br /><p align="justify"><br />The research result shows that the best extraction method for EPS extraction from microorganisms in the activated sludge was NaOH Extraction method. The amount of the polysaccharides obtained was 18.09 mg EPS/g TSS. On other hand, Steaming Extraction was the most ineffective extraction method. This method gives the polysaccharides result in the least amount, i.e., about 4.96 mg EPS/g TSS. In this research, the protein content was not detected in the all used EPS solution.<br /><p align="justify"><br />In the adsorption test, the adsorption phenomena of copper metal with EPS adsorbent fitted to the Freundlich isotherm adsorption equation. The values of Freundlich's a constant (k and n) each were 2.282 and 0.963. Value of R² as a result of a plotting Cu residue concentration and the adsorption capacity data at equilibrium adsorption condition to the linearized Freundlich equation was 0.905. The average maximum adsorption capacity of EPS to metal Cu was 88.34 mg Cu for each gram of EPS.<br /><br />