OPTIMIZATION OF EXPLANT STERILIZATION METHODS IN TISSUE CULTURE OF BLACK BAMBOO (GIGANTOCHLOA ATROVIOLACEAE WIDJDJA) AND HAUR BAMBOO (BAMBUSA TULDOIDES MUNRO)

• Main Author: Meidina, Faza
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62101
• Institution: Institut Teknologi Bandung

G. atroviolacea and B. tuldoides are endemic bamboo species to Indonesia and have enormous potential to be developed. The quality of bamboo affects the product produced, it can be started by choosing a seedling method. Technically, one method of propagation that will produce uniform seeds in large numbers is tissue culture. The problem with tissue culture is the presence of contaminants. One way to control it is by sterilization techniques. The purpose of this study was to determine the effect of soaking time of HgCl2 and the addition of sterilant on the sterilization technique of G. atroviolacea, and B.tuldoides, determine the ratio of the average percentage of survival between G. atroviolacea, and B.tuldoides, and determine the appropriate treatment to suppress bacterial contamination. , fungus and browning in the sterilization technique of G. atroviolacea, and B. tuldoides. The results showed that: 1) there was no effect of soaking time of HgCl2 on bacterial contamination, fungal contamination and browning. While the sterilant has an effect on bacterial contamination and fungal contamination but has no effect on browning; 2) G. atroviolacea had the highest survival percentage of 58% while B. tuldoides died in the 4th week; 3) The treatment of HgCl2 + povidone iodine was the right combination of sterilants for G. atroviolacea by producing the lowest bacterial contamination percentage of 2.08% and the lowest fungal contamination of 25%. The use of HgCl2 is the right sterilant for B. tuldoides to suppress the lowest bacterial contamination of 19.79% and HgCl2+povidone iodine to suppress the lowest fungal contamination of 25%.