PENGARUH PEMBERIAN EKSTRAK BUNGA CHAMOMILE (MATRICARIA RECUTITA L.) TERHADAP KADAR GLUKOSA DARAH MENCIT (MUS MUSCULUS L.) YANG DIINDUKSI ALOKSAN

• Main Author: Gloria Feriani, Kania
• Format: Final Project
• Language: Indonesia
• Subjects: Ilmu hayati ; Biologi
• Online Access: https://digilib.itb.ac.id/gdl/view/62113
• Institution: Institut Teknologi Bandung

Diabetes is a disease characterized by high levels of glucose concentration in the blood (hyperglycemia) due to the body's inability to produce enough insulin or the body's inability to use insulin effectively. Handling diabetes mellitus is generally done by giving synthetic drugs which are not free from side effects such as diarrhoea, nausea, and weight gain. To reduce these side effects, people with diabetes mellitus generally turn to alternative treatments such as medicinal plants that have the potential as antidiabetic. One of the plants that can be trusted to control blood glucose levels in people with diabetes mellitus is high levels of antioxidants in the form of phenolic and terpenoid compounds in chamomile plants. Phenolic compounds (flavonoids) are mostly in the flower (capitulum) of chamomile. Research on the effect of chamomile plants on blood glucose levels has occurred directly and through various dosage tests, it was found that chamomile plants have great potential as a natural anti-diabetic treatment. However, testing with the multilevel dose method is still very few and generally multilevel doses are made from the entire chamomile plant consisting of flowers, stems, leaves, and aerial areas. Based on these reasons, this study aimed to determine the effect of chamomile flower grade extract on blood glucose levels conditioned by diabetes mellitus with the four doses tested significantly lowering blood glucose levels conditioned by diabetes mellitus. This study used an experimental method of pre and posttest control group design using 24 male mice aged 2 months with a bodyweight of 20-25 grams, which were then divided into 6 treatment groups, namely normal control (healthy mice), negative control (Diabetic mice), dose 1 (13 mg/kg body weight mice), dose 2 (26 mg/kg body weight mice), dose 3 (52 mg/kg body weight mice), and dose 4 (104 mg/kg body weight mice). Mice were conditioned to diabetes mellitus by injecting alloxan 70 mg/kg BW in mice via the caudal vein until their blood glucose levels were at least in the range of 200-349 mg/dl. Checking blood glucose levels were taken via the caudal vein once every 3 days for 19 days after previously the mice fasted for ±18 hours. Then the research data were analyzed by using paired sample t test and repeated measures anova. Based on the results of the paired sample t test, it was found that the administration of alloxan compounds could significantly increase the blood glucose levels of mice. Then based on the results of the repeated measures anova test, it was found that the administration of chamomile extract could significantly reduce the blood glucose levels of mice. The case of a decrease in blood glucose levels can occur because chamomile can suppress the activity of ROS (reactive oxygen species) so that pancreatic beta cells become more sustainable. The conclusion of this study is that chamomile flower extract can significantly reduce blood glucose levels in mice, although it does not reach normal conditions with the dose that has the most potential as an antidiabetic treatment is dose 4 (104 mg/kg BW mice).