TELAAH FITOKIMIA DAUN BINAHONG [ANREDERA CORDIFOLIA (TEN.) V. STEENIS]
Binahong [Anredera cordifolia (Ten.) v. Steenis, Basellaceae] leaf is traditionally used for treating many kinds of diseases, however, the data of its chemical content is still limited. This research is aimed to study chemical content of binahong leaf and to determine identity compound of its cru...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/62160 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Binahong [Anredera cordifolia (Ten.) v. Steenis, Basellaceae] leaf is traditionally used for
treating many kinds of diseases, however, the data of its chemical content is still limited.
This research is aimed to study chemical content of binahong leaf and to determine identity
compound of its crude drug. Phytochemical screening showed a presence of flavonoid,
saponin, and steroid/triterpenoid compounds. Crude drug was macerated with ethanol and
the extract was fractionated through liquid-liquid extraction. Two flavonoid compounds
were isolated from ethyl acetate fraction and the isolates were purified by preparative paper
chromatography and characterized using spray reagent of aluminum(III) chloride 5% and
ultraviolet-visible spectrophotometry with the use of shift reagents. The isolates had
ultraviolet-visible spectrum data as flavones with OH-group at positions of C 5, 7 and 4’.
The flavones were an aglycone and its glycoside which could be used as identity
compounds of the ethanol extract of binahong leaf. By using paper chromatography system
with acetic acid 15% as mobile phase and visualized under UV light 366 nm with the use
of spray reagent aluminum(III) chloride 5%, both compounds were shown as yellow
fluorescent spots with Rf values of 0.25 and 0.5.
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