PEMBENTUKAN KOMPLEKS NIKEL - KLORAMFENIKOL DAN UJI AKTIVITAS ANTIBAKTERINYA TERHADAP STAPHYLOCOCCUS AUREUS, ESCHERICHIA COLI, METHICILLIN RESISTANT STAPHYLOCOCCUS AUREUS (MRSA) DAN VANCOMYCIN RESISTANT ENTEROCOCCUS (VRE)

• Main Author: Ningsih, Listia
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62180
• Institution: Institut Teknologi Bandung

Chloramphenicol (CHL) is a broad spectrum antibiotic which rarely used on human because of its high toxicity level and bacteria resistance. Complexes synthesis through covalent coordination bonding can enhance the activity of drugs, included antibiotic. Based on the recent studies, metal complexes formed from CHL with Co(II) and Fe(II), showed higher antibacterial activity. Study of metal complexes from Ni(II) and CHL is unknown.This study objective is to synthesis Ni-CHL metal complexes and test the antibacterial activity of the complexes against S. aureus, E.coli, MRSA dan VRE. Ni- CHL complexes were made by using pH adjustment method crystalisation. Obtained crystal are analyzed and characterized by using FTIR and H-NMR. Furthermore, the antibacterial activity was tested by using agar diffusion method against S. aureus, E.coli, MRSA dan VRE. Formed inhibition zone from the complexes was compared with inhibition zone from mixed compound and CHL. Complexes crystal which was made by pH adjustment method had needlelike form and yellowish green colour. FTIR spectra indicated that spectra of –OH and nitrophenyl groups were shifted to 3347 and 1346 cm -1 . A new peak was found at 698 cm -1 region from stretching C-Cl spectra. H-NMR spectra showed peak shift from hidroxyl (-OH) from 1,3-propanediol groups and dichloramide from CHL. Ni-CHL inhibition zone, mixed compound and CHL against S.aureus were 24.75; 21.86 and 23.86 mm. Ni-CHL inhibition zone, mixed compound and CHL against E.coli were 34.58; 31.89 and 34.07 mm. Ni-CHL inhibition zone, mixed compound and CHL against MRSA were 28.04; 27.03 and 29.93 mm. Ni-CHL inhibition zone, mixed compound and CHL against VRE were 27.88; 26.76 and 27.66 mm. Ni-CHL complexes were formed by using pH adjustment method. Antibacterial activity of complexes was significantly different (p<0.05) compared to antibacterial activity of mixed compound and CHL against S. aureus with 13% dan 5% enhace activity level. Antibacterial activity of complexes was not significantly different (p>0.05) compared to antimicrobial activity of mixed compound and CHL against E. coli , MRSA and VRE.