KARAKTERISASI ENZIM LIPASE PROPIONIBACTERIUM ACNE YANG DIISOLASI DARI JERAWAT WAJAH

• Main Author: Victoria Sugianto, Felita
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62182
• Institution: Institut Teknologi Bandung

Acne is a chronic inflammation of the sebaseous follicles of the skin characterized by comedones, papules, pustules and nodules. One of the factors that influence the development of acne is the presence of bacterial colonization on pilosebaseous gland. Propionibacterium acne is one of the bacteria that play a role in the pathogenicity of acne that can produce proinflammatory cytokines such as antigen that can trigger inflammation as the response of cellular and noncellular immune. The enzyme of extracellular lipase which is produced from Propionibacterium acne is able to hydrolyze triglycerides sebum produces by the sebaceous glands into free fatty acids. This research aims to determine characterization of the enzymes of lipase Propionibacterium acne. The identification and isolation of bacteria from five samples (A, B, C, D, E) of patients with acne have been done. Crude enzyme was obtained after the isolation of pure cultures of Propionibacterium acne with supernatant as a solution of enzyme. Gum solution consist of gum arabic made by the cone of emulsion with the addition of buffer with certain pH. Substrate emulsion as a blank consist of gum solution followed by the addition of olive oil as substrate. Substrate emulsion that was reacted with enzyme solution was incubated at the optimum temperature and time so as to produce a marked decrease in turbidity with a decrease in absorbance using a spectrophotometer visible at a wavelength of 600 nm.The optimization of lipase was at pH 4-9 with a temperature 20-60 o C and the incubation time was 10-60 minutes. Constanta Michaelis-Menten (Km) and maximal velocity (Vmax) as parameter were determined to obtain characterization of enzyme of lipase Propionibacterium acne. Propionibacterium acne was found in the acne samples of patients B, C, and D. The optimum of enzyme of lipase was at pH 6, temperature 30 o C, and the incubation time of 30 minutes. Constanta parameter Michaelis-Menten (Km) the patient’s samples B, C, and D respectively were 0.043, 0.026, 0.035 g/mL. Vmax values of lipase from the patient’s samples B, C, and D in a row was equal to 304.85 minutes -1 , 572.04 minutes -1 ; 268.65 minutes -1 .