UJI EFEK IMUNOSTIMULAN DAN HEPATOPROTEKTOR EKSTRAK DAUN ASAM JAWA [TAMARINDUS INDICA L.] PADA MENCIT SWISS-WEBSTER BETINA

• Main Author: Safithri S, Amanda
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62198
• Institution: Institut Teknologi Bandung

Tamarind leaves have been traditionally used to cure fever, cough, gout, jaundice, worm infection, ulcers, boils, and insomnia. The hepatoprotective effect of tamarind leaves extract had been proven in a research using chemical-induced hepatotoxicity method. The objective of this research is to study immunostmulatory and hepatoprotective effect of tamarind leaves extract. Crude drugs was extracted by infusum method. Water extract was used to test immunostimulatory and hepatoprotective effect. Evaluation of immunostimulatory effect was divided into non specific immune responses (carbon clearence and organ index) and specific immune responses (antibody titer and delayed hipersensitivity type). The evalution was done in mice that were given tamarind leaves extract in doses of 250 mg/kg bw, 500 mg/kg bw, 1000 mg/kg bw, and zymosan A as standard drugs in 7 days. Two doses of highest immunostimulatory activity was further evaluated for hepatoprotective effect. The decrease of SGOT, SGPT, and total bilirubin in carbon tetrachloride- induced mice was measured on the 2 nd day, 5 th day, and 8 th day after induction. Tamarind leaves extract and silimarin as standard drug were given 7 days before carbon tetrachloride induction. Organ index and liver histopatology were also done to evaluate hepatoprotective effect. Based on Weigner index, the 250 mg/kg bw dose showed moderate imunostimulatory activity while the 500 mg/kg bw and 1000 mg/kg bw doses showed potent imunostimulatory activity. The primary antibody titer showed that there was an increase antibody titer at doses of 500 mg/kg bw and 1000 mg/kg bw compared to that of normal group. At secondary antibody titer, all of test groups showed an increase in antibody titer compared to normal group and zymosan A. Delayed type hipersensitivity showed that there were significant differences (p < 0,05) in foot inflammation at dose of 1000 mg/kg bw compared to that of normal group at T48. Results indicated that tamarind leaves extract have immunostimulatory effect. Two doses were recommended to be used in hepatoprotective effect evaluation i.e 500 mg/kg bw and 1000 mg/kg bw, showing significant decrease (p < 0,05) of SGOT, SGPT, and total bilirubin. Microscopic analysis in liver histology showed the presence of necrosis at liver cells in positive group, 1000 mg/kg bw, and silimarin at different damaged levels. Tamarind leaves extract at doses of 250 mg/kg bw, 500 mg/kg bw, and 1000 mg/kg bw showed immunostimulatory effect. The doses of 500 mg/kg bw and 1000 mg/kg bw also showed hepatoprotective effect.