PEMODELAN KINETIK PERTUMBUHAN DAN PRODUKSI LIPID DALAM KULTUR SPIROGYRA SP. YANG DIBERI PENAMBAHAN NATRIUM KLORIDA

• Main Author: Putri Safira, Nabila
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62316
• Institution: Institut Teknologi Bandung

Spirogyra sp. is known to contain 11-21% dry weight (DW) lipid and these algal lipid can be utilized for various industries. Lipid content in algae can be enhanced by growing the algae under different salt concentrations. Therefore, the objective of this study was to determine the effect of various NaCl concentrations on growth and lipid content of Spirogyra sp. and to determine the optimum NaCl concentration for the highest lipid productivity. Spirogyra sp. was grown in modified Bold Basal Medium (BBM) with an addition of NaCl at various concentrations i.e. 0.025; 0.05; and 0.075 g L-1 for 8 days. The kinetic of the growth and lipid production of Spirogyra sp. were determined using logistic and Luedeking-Piret model. The results showed that the highest biomass growth was obtained under 0.05 g L-1 NaCl with maximum biomass (Xmax) i.e. 344.93 mg L-1 and maximum specific growth rate (?max) i.e. 0.153 day-1. On the other hand, parameter estimation from logistic model showed that the highest Xmax (338.4 mg L-1) was obtained under 0.075 g L-1 NaCl and the highest ?max (1.017 day-1) was obtained under 0.05 g L-1 NaCl. This study indicated that the addition of NaCl could enhance the lipid content in Spirogyra sp. with the highest lipid yield of 90.71 mg g-1 was obtained under 0.075 g L-1 NaCl. Parameter estimation from Luedeking-Piret model showed the value of ? and ? ? 0, this result indicated that lipid production in Spirogyra sp. was partially associated with growth. The highest lipid productivity of 8.79 mg g-1day-1 was obtained under 0.075 g L-1 NaCl. This result showed that lipid productivity increase up to 52% compared to the control.