SECONDARY METABOLITES OF THE POLAR FRACTION OF ENDOPHYTIC FUNGUS FUSARIUM SOLANI ASSOCIATED WITH THE PLANT CRYPTOCARYA PULCHRINERVIA

• Main Author: Ali, Moh.
• Format: Theses
• Language: Indonesia
• Subjects: Kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/62588
• Institution: Institut Teknologi Bandung

Cryptocarya is one of the largest genus belonging to the Lauraceae family, this plant is at the most advanced stage of evolution among the other genus in the Lauraceae family. Based on phytochemical studies, this genus contains major secondary metabolites, namely alkaloids, flavonoids and ?-pyrons and also contains minor secondary metabolites such as stilbenes, lignans, steroids and terpenoids. Based on their bioactivity, some of these compounds are reported to be potential as anticancer, antitumor, cytotoxic, antibacterial and antiviral. The demand for various types of drug is increasing, but the main compound for drugs derived from plants are limited. Therefore, one alternative that can be used as a source of bioactive compounds other than plants is endophytic fungi. Endophytic fungi living in their host plant tissues are capable of producing and engineering various secondary metabolites to increase the host's growth rate in competition and self-defense in their environment. This study aims to conduct a phytochemical study of the polar fraction of endophytic fungi from plant tissue of C. pulchrinervia, which includes isolation and characterization of endophytic fungi from C. pulchrinervia branch, then isolation and characterization of secondary metabolites from the polar fraction of the endophytic fungi EtOAc extract and conduct a bioactivity test of the isolated compound against P-388 murine leukemia cells and microbes. The isolation of endophytic fungi from C. pulchrinervia branch was done through surface sterilization, inoculation and subculture stages using PDA (Potato Dextrose Agar) media to obtain a single fungal isolate. The study results of molecular identification based on the Internal Transcribed Spacer (ITS) genetic analysis of fungal DNA showed that the single fungal isolate was Fusarium solani. Endophytic fungus F. solani was cultivated and incubated in rice medium for 28 days (28o C). 7 grams of EtOAc extract were collected from the total of 40 erlenmeyer rice media used for cultivation. Furthermore, to obtain pure compounds from the polar fraction of the EtOAc extract, fractionation and further purification were carried out using various chromatographic techniques, namely vacuum liquid chromatography (KCV), gravity column chromatography (KKG) and radial chromatography (KR). Three pure compounds were obtained from the purification stage. The three pure compounds were characterized based on 1D (1H and 13C-NMR) and 2D (HSQC and HMBC) NMR spectroscopic data. One of the pure compounds with code AL1 was identified as fusarin C derivative compound and the other two pure compounds were still structurally unidentified. The results of the antibacterial activity test against the gram-positive bacteria Stenotrophomonas rhizophila and the gram-negative bacteria Enterobacter aerogenesis showed that the compound 13?–Hydroxylucilactaene (AL1) strongly inhibited the bacteria S. rhizophila with MIC value = 4 µg/mL and weakly inhibited the bacteria E. aerogenesis with MIC value = 512 µg/mL.