PENCIL GRAPHITE ELECTRODE (PGE) MODIFICATION USING MOLECULARLY IMPRINTED POLY(GLYCINE) FOR VOLTAMMETRIC DETECTION OF SUNSET YELLOW
Sunset yellow is synthetic dye mostly containing azo functional group and aromatic structure. In Indonesia this synthetic dye is not banned with maximum level 4 mg/Kg weight. Mostly SY is used for coloring foods and beverages. Thus, monitoring or controlling is needed. SY detection method is ineffic...
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| Format: | Theses |
| Language: | Indonesia |
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| Online Access: | https://digilib.itb.ac.id/gdl/view/62717 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Sunset yellow is synthetic dye mostly containing azo functional group and aromatic structure. In Indonesia this synthetic dye is not banned with maximum level 4 mg/Kg weight. Mostly SY is used for coloring foods and beverages. Thus, monitoring or controlling is needed. SY detection method is inefficient, costly, and complicated procedure. SY determination needs fast, low cost, simple and accurate analysis method. Voltammetric method is applicable for SY determination due to electroactive species. Pencil graphite electrode (PGE) is categorized as lack of development although having many advantages such as economist, easy to find, and simple. However, PGE is still less of selective and sensitive. Thus, in this research determination of SY was evaluated using PGE modified by SY imprinted polyglycine. The aim of this research is modification of PGE that become more selective and sensitive which can use for real sample. PGE-MIP was investigated by measuring SY 0,1 mM in PBS 0,1M pH 8 using SWV technique within 400-1000 mV frequency 25 Hz. This research was studied about determination of analyte measuring technique, peak current evaluation, MIP composition ratio analyte - polymer effect, electropolymerization cycle effect, scan rate and pH effect. Peak current as respon of SY was measured by PGE-MIP which is higher than PGE-NIP and PGE-bare. Proposed PGE-MIP was good both repeatability and reproducibility (7,14%;4,11%). This PGE-MIP has excellent of dynamic range from 1-10 µM with LOD 0,549 µM. Electrochemical adsorption mechanism was involved on surface of PGE-MIP. Recovery percentage of PGE-MIP was good (98.86%). PGE-MIP is selective electrode for SY which endure of interference such as NaCl, benzoic acid and glucose. The result of SY determination using PGE-MIP (voltammetric method) was not significantly different comparing with UV -VIS spectrophotometric method (?=482 nm). |
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