PRODUKSI BIBIT DENGAN KULTUR PUCUK TANAMAN PISANG (MUSA ACUMINATA CV CAVENDISH) MENGGUNAKAN BIOREAKTOR TIS RITA ®

• Main Author: Handianto, Adrianus
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62985
• Institution: Institut Teknologi Bandung

Cavendish banana (Musa acuminata) is a fruit commodity that is very common and has the potential to support food security in Indonesia. Optimization of the banana seedling production process can be done by using tissue culture techniques with larger number of cultivation results and faster seed maturation time. The Bioreactor Temporary Immersion System (TIS) is a system that combines the advantages of using a liquid medium, but avoids continuous immersion so as to provide aeration and agitation of nutrients evenly. In this study, growth and economic analysis will be analyzed by calculating the survival rate of Cavendish banana shoot culture in a liquid system and TIS-RITA® bioreactor with variations in immersion time of 5 and 20 minutes using Murashige & Skoog liquid medium half-strength and gibberellic acid 0,5 ppm. The growth of shoot culture was determined by calculating the growth rate of biomass. Survival rate is determined by growing plants ex vitro. The average growth rate of Cavendish banana shoot culture at 5 minutes and 20 minutes soaking time variation was 0.05 cm/day and 0.053 cm/day, respectively. In the liquid system, the survival rate obtained was 66.7%, while in the TIS-RITA® bioreactor it was 100%. The net present value of the application of TIS-RITA® bioreactor technology can reach Rp. 13,796,000.00, while the liquid system has a net present value of Rp. 11,990,670.00. The payback period for TIS-RITA® required is 2 years and 5 months, which is a little longer than the payback period for thin-layer culture, which is 2 years.