PRODUKSI PROTEIN DAN BIOMASSA DARI KULTIVASI SPIRULINA PLATENSIS DAN CHLORELLA VULGARIS DENGAN MEMANFAATKAN LIMBAH CAIR PERIKANAN SEBAGAI MEDIUM KULTIVASI DAN VARIASI PERLAKUAN MIXED CULTURE

• Main Author: Yudha Prawira, Muhammad
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/62996
• Institution: Institut Teknologi Bandung

The world's food needs will continue to increase along with the increasing human population. Limited availability of protein and essential vegetable oils is a challenge in meeting nutritional needs. Therefore, it is necessary to have alternative protein sources to answer these problems, one of which is microalgae. Microalgae are known to have a lot of potential, ranging from waste remediation, development of aquaculture systems, to the production of various bioproducts such as biodiesel, functional food, food coloring, and animal feed. Unfortunately, the high operational costs of microalgae cultivation, mostly due to the high cost of synthetic growth media, can hinder the production of protein and essential oils of microalgae on an industrial scale. One alternative medium that has the potential as a medium for growing microalgae is the aquaculture wastewater. This study aimed to determine the effect of single culture of Spirulina platensis, single culture of Chlorella vulgaris, and mixed culture of Spirulina platensis and Chlorella vulgaris on the levels of inorganic compounds in the growing medium of aquaculture wastewater by observing the protein and chlorophyll content in microalgae. In this study, mixed culture was carried out with a ratio of 1:1 (v/v) for Spirulina: Chlorella culture with a cultivation time of 7 days. The cultivation treatment with the highest specific growth rate was the 1:1 Mixed cultivation of 0.511 ± 0.006. The highest protein content was obtained at 1:1 Mixed cultivation of 93.926 ± 1.816 mg/g wet biomass. The treatment variation with the highest total chlorophyll content was obtained in a single culture of Spirulina platensis of 17.943 ± 0.341 mg/L. The highest percentage and rate of nitrate reduction was found in the single culture variation of Spirulina platensis of 21.305% and 0.083 mg/L day, respectively. The highest percentage of ammonium reduction was obtained from the 1:1 Mixed cultivation variation of 90.909%, while the highest ammonium reduction rate was obtained by single culture of Spirulina platensis of 0.13 mg/L day. The 1:1 Mixed cultivation treatment had the highest percentage and rate of orthophosphate reduction of 16.308% and 0.235 mg/L day.