SYNTHESIS AND CHARACTERIZATIONS OF IRON NANOPARTICLES VIA CHEMICAL REDUCTION METHOD FOR BIOSENSOR APPLICATION

• Main Author: Artha Febriyanti Turnip, Maria
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/63098
• Institution: Institut Teknologi Bandung

Metal nanoparticles with a size smaller than 100 nm are widely applied in various fields, one of which is in the biomedical field, namely biosensors. A biosensor is a device that measures biological or chemical reactions resulting from interactions between bioreceptors (specific molecules that recognize analytes) and analytes (biological materials obtained from an infected or contaminated immune system). In this research, we study the synthesis of iron nanoparticles by use of the chemical reduction method to produce biocompatible iron nanoparticles for biosensor applications. This chemical reduction method uses citric acid as a reducing agent and its capping material and iron(III) nitrate as a source of iron. The iron nanoparticles produced are in the form of a yellow solution and have a pH of 2. The iron-citric acid nanoparticle experiment resulted in iron nanoparticles with an average size of 100.7 nm and spherical shapes that stick to each other. While the UV Vis spectrum shows a wide absorbance region at 273 nm. The results of SEM show the composition of the dried iron nanoparticles consisting of oxygen and iron. Then added ammonia to adjust the pH to around 6-7 and obtained iron nanoparticles with an average size of 176.5 nm. The TEM results show iron nanoparticles that form aggregations. Meanwhile, the UV Vis spectrum showed a wide absorbance region at 285.5 nm and 455.11 nm. To utilize iron nanoparticles for biosensor applications, a synthesis of iron nanoparticles was carried out by capping 3-mercaptopropionic acid (3-MPA) which has two functional groups, namely sulfur and carboxylic acid (COOH). This COOH group is very flexible to be able to bind to biological molecules such as proteins, glucose, and others that are specific for binding to the analyte in the biosensor system. The resultant iron-MPA nanoparticles have formed aggregations and have a wide absorbance region at 268 nm. In addition, the synthesis of iron nanoparticles with a reducing agent trisodium citrate was also carried out. The resulting iron nanoparticles are spherical with an average size of 76.97 nm and an absorbance area of 279 nm. The absorbance region at 270-280 nm is characteristic of the iron nanoparticles and their aggregations formed in the solution.