#TITLE_ALTERNATIVE#

• Main Author: Ayu R. K. (NIM 253 04 042), Ketut
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/6358
• Institution: Institut Teknologi Bandung

Abstract : <br /> <br /> <br /> <br /> <br /> The halophilic culture was established with benzene as the sole carbon source by using a sea sediment from estuary of Mahakam River. The primary objective of this work was to evaluate the ability of halophilic bacteria present in the sea sediment from estuary of Mahakam River to degrade benzene. Benzene is most problematic, because it is highly water soluble, the most stable aromatic compound, is a known carcinogen and toxic. Benzene may contaminate the environmental as a result of petroleum industrial activities. Biodegradation of benzene in brackish water have special characteristic due to effect of high salinity and activity of special group of microorganism. The biodegradation kinetic parameter for benzene are provided from laboratory experimental in an semi batch reaktor. The calculation of biodegradation of the observed compound was based on the removal of initial concentration of effluent discharge. The nutrient and trace mineral come from the mineral content in seawater. The sea sediment provides a wide spectrum of halophilic microorganism which responsible for biodegradation process. The substrate for microorganism growth are benzene. The assay performed in two parallel experiments with different concentration. The result showed that the culture completely degraded benzene within 2 weeks. The kinetic study showed that degradation rate in sea water for Reaktor 1 was 0.1272 mg/l.day and Reaktor 2 was 0.111 mg/l.day. The degradation of benzene in the sediment in Reactor 1 was 0.3852 mg/kg.day and Reaktor 2 was 0.2774 mg/kg.day. Community structure analysis revealed that Pseudomonas sp, Pseudomonas diminuta, Pseudomonas putida, Bacillus sp and Bacillus spaericus were the dominant members of the culture. <br />