#TITLE_ALTERNATIVE#

• Main Author: Ajeng Pitayu (NIM 107 03 055), Laras
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/6386
• Institution: Institut Teknologi Bandung

Abstract : <br /> <br /> <br /> <br /> Superoxide Dismutase (SOD) is an enzyme naturally produced by organisms consuming oxygen. SOD plays one role in defense mechanism against reactive oxygen species which is produced as a metabolism and respiration by products. In this study, screening of SOD activity was carried out to 14 unidentified marine and soil bacteria isolated from Indonesia. After obtaining a number of bacteria with high SOD activity, identification of their species using 16S rDNA was done. Gram staining and observation of cell morphology were done to 29 bacteria and 14 of them were randomly selected and screened for the activity of SOD enzyme. The activity was determined based on the value of reduction inhibition percentage, which is a ratio of absorbance of bacterial total protein and that of solution containing only NBT. The higher the SOD activity in protein extract, the lower intensity of blueish violet color produced. After obtaining protein extracts with the highest activity, PCR 16S rDNA followed by sequencing was done to identify the species of SOD producers. Six protein extracts with highest activity i.e. from SEDAL cle II3, Seskau 2, TK Cibodas, MKS 11, MKS 13 and Ciismun 4 bacteria were obtained with their values of reduction inhibition percentage were 25,15 %, 34,66 %, 35,75 %, 36,31 %, 37,5 %, and 38,67%, respectively. From nucleotide sequences of their 16S rDNAs, four species were identified i.e. Bacillus subtilis, Enterobacter cloacae, Shigella boydii, and Escherichia coli. <br />