KINETICS OF THE LIPOLYSIS REACTION OF TRIGLYCERIDE USING IMMOBILIZED LIPASE ONTO CELLULOSE NANOCRYSTALS (CNCS)

• Main Author: Arabica Yatasya H, Fauzatu
• Format: Theses
• Language: Indonesia
• Subjects: Teknik kimia
• Online Access: https://digilib.itb.ac.id/gdl/view/64172
• Institution: Institut Teknologi Bandung

Fatty acids are important commercial commodity because widely used by industrial and public needs. Over the past decade, lipolysis for fatty acids production is being developed because it requires much lower energy and lower risk of product degradation. However, the disadvantage of this process is the high price of enzymes. The operating costs of this enzymatic hydrolysis processes can be reduced by using immobilized lipases. The kinetics of immobilized lipase as bionanocatalyst for lipolysis reaction needs to be analysed to ensure its performance in the triglyceride lipolysis application to produce free fatty acids. This research aims to synthesize lipase-CNCs (Cellulose Nanocrystals) through lipase immobilization as a catalyst for lipolysis reaction. The process immobilization in this research was carried out using covalent bonding method. The kinetics parameter of lipolysis reaction was studied by varying the stirring speed of 50–200 rpm, palm oil fractions of 10–50%-v/v, and reaction temperature of 30–45°C. The initial study of the characterization of CNCs from CelluForce confirmed that the materials used were CNCs with degree of crystallinity (CrI) of 75.6%, needleshaped morphology with average length of 125±13 nm, and had a carboxylate functional group. In addition, the result indicated that the optimum emulsifier concentration for the lipolysis reaction of triglycerides was 5%-w/v. The optimum stirrer speed which causes external mass transfer does not affect the reaction rate is 200 rpm. Furthermore, the Michaelis-Menten kinetic parameters (Km and Vm) for lipolysis with free lipase were 70.79 ?mol/mL and 50.25 ?moL/(mL.hour) respectively. Meanwhile, the Km and Vm values for lipolysis with immobilized lipase were 112.98 ?mol/mL and 12.11 ?moL/(mL.hour) respectively. The activation energy of triglyceride lipolysis using immobilized lipase as a catalyst was 33.5 kJ/mol.