FORMULASI NANOEMULSI JAKALIN DART BIJI NANGKA (ARTOCARPUS HETEROPHYLLUS L.) SEBAGAI ADJUVANT MELALUI RUTE TRANSDERMAL

• Main Author: Ervinda, Sarah
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/64290
• Institution: Institut Teknologi Bandung

Vaccine generally formulated by the addition of immune response enhancer (adjuvant) and administered via parenteral route. However, there are some disadvantages from giving vaccine and adjuvant through this route of administration. The purpose of this research was to develop a non¬invasive vaccine formulation via transdermal route with BSA (Bovine Serum Albumin) subunit protein vaccine model using jacalin as the adjuvant. Jacalin was obtained from the isolation process of jackfruit seeds (At-focal-pus heterophyllus). Nanoemulsion formulated using self-nanoernulsification method with the oil in water type of emulsion. Formulations were varied including the concentration of lecithin in lyophilisation process with BSA and Jacalin, concentration of surfactant and co- surfactant, concentration of oil and water. Evaluations of nanoemulsion included physical and chemical evaluation. Physical evaluation consists of the measurement of globule size, polidispersity index, viscosity, zeta potential, and stabilty test with centrifugation. Chemical evaluation consists of pH measurement, entrapment efficiency, hemoaglutination test, and diffusion test. Stable nanoetnulsion was obtained from the formulation consist of 2.5% oleic acid, 21% tween 80, 21% propylene glycol, and 55.5% water. The optimum ratio of lecithin with BSA and jacalin in liophillisation process was l :3. Nanoemulsion had an average size distribution of 60.2 ± .,57 nm with polydispersity index of 0. 152 ± 0.26 and zeta potensial 1.08 ± 0.12 mV. Entrapment efficiency of nanoemulsion was more than 97.5%. Jacalin showed hemoaglutination activity at concentration of 2.5 ppm. Based on diffusion test, the cumulative amount of BSA penetrated through snake's skin after 8 hours was 53.44 ± 5.07 %. The nanoemulsion obtained from this research had a good characteristic based on the result of evaluation process. Hemaglutination assay showed jacalin still had activity in nanoemulsion dosage form. The globule size in nanoemulsion was less than .100 nm and nanoemulsion had efficient entrapment efficiency. Nanoemulsion had potential for the application of transdermal vaccine.