FORMULASI LARUTAN KONSENTRAT EKSTRAK RIMPANG TEMULAWAK (CURCUMA XANTHORRHIZA ROXB.) DAN DAUN NIMBA (AZADIRACHTA INDICA A. JUSS.) UNTUK UNGGAS

• Main Author: Liliani
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/64295
• Institution: Institut Teknologi Bandung

Background and purpose: Java turmeric has been traditionally used in treating liver disorders. Neem also has been used as hcpatoprotector. Liver is one of the important organs in human being and animal which has functions such as toxin detoxification and metabolic regulation. The objective of this experiment was to formulate concentrated solution of Java turmeric and neem to prevent and treat liver disorder. Method(s): The dry Java turmeric and neem were extracted using percolator with methanol as solvent. The chemical content of dry Java turmeric and concentrated extract was characterized by GF254 TLC with eluent chloroform-methanol-glacial acetic acid (95:5:1), while the chemical content of dry neem and concentrated extract was characterized by GF254 TLC with eluent choloroform-ethyl acetate (9:1). The chromatograms were observed under visible light, UV light of 254 nm, UV light of 366 nm, and spray reagent H2SO4 10% in methanol. The formula of concentrated solution of Java turmeric and neem used sorbitol or glycerin or propylene glycol and with or without polysorbate 80. Evaluation of the concentrated solution included organoleptic, pH, qualitative dissolution, and bitterness value tests. Long term and accelerated stability tests were performed at room temperature and at 40°C with humidity 75% respectively. Quantitative determination of curcuminoid was conducted by TLC densitometry with detector wavelength 420 nm and 366 mu for neem marker compound. Stability test was perfomed by TLC densitometry to determine curcuminoid concentration at 420 nm of wavelength and compare the AUC result of neem marker compound at 366 nm of wavelength. Result: The formula which consisted of 10% Java turmeric extract, 10% neem extract, 0.5% polysorbate 80, and propylene glycol gave a dosage form with no precipitate and not separated before and after centrifugation. The reduction velocity of curcuminoid concentration at room temperature and 40°C were 1.386 ppm per 14 days and 1.8975 ppm per 14 days respectively. Conclusion: The result of sample extrapolation showed that 95% curcuminoid concentration at room temperature would be reached after 74 months.