PENGEMBANGAN FORMULA NANOEMULSI SPONTAN DENGAN PEMANFAATAN JAKALIN DARI BIJI ARTOCARPUS INTEGRIFOLIA SEBAGAI ADJUVAN VAKSIN TRANSKUTAN
Vaccine is a preparation containing antigenic compounds to provide specific immunity in humans. One of barriers to ensure the effectiveness of vaccine preparation is the safety and effective delivery. At present, parenteral route is commonly used for vaccine delivery that is known having some drawba...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/64299 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Vaccine is a preparation containing antigenic compounds to provide specific immunity in humans. One of barriers to ensure the effectiveness of vaccine preparation is the safety and effective delivery. At present, parenteral route is commonly used for vaccine delivery that is known having some drawbacks. Alternatively, the vaccine can be administered transcutaneous. The objective of this research was to develop nanoemulsion formula in the form of transcutaneous vaccine. Bovine serum albumin (BSA) is used as a model protein of antigen and jacalin isolated from the seeds of jackfruit (Artocarpus integrifolia), was made in solid dispersion form with lecithin. Nanoemulsion was prepared using corn oil as lipophilic phase and stabilized by Tween 80 as surfactant and glycerin as cosurfactant. BSA and jacalin was made as solid dispersion form with lecithin to improve their dispersibility in oil. Nanoemulsion was evaluated by measuring of globule size and distribution, zeta potential, BSA entrapment efficiency, hemaglutination activity of jacalin, and conducting physical stability testing by Freeze and Thaw. The optimum formula of nanoemulsion consisted of corn oil, Tween 80, glycerol, and BSA solid dispersion in the ratio of 3%, 15%, 15%, and 1%, respectively. The average particle size was 51.5 ± 8.82 nm with polydispersity index of 0.269 ± 0.029 and zeta potential of 0.066 ± 0.015 mV. Protein entrapment efficiency in the nanoemulsion was 99.70 + 0.053%. Hemaglutination activity test of BSA-jacalin nanoemulsion was seven times lower than isolated jakalin solution. The physical evaluation consisted of average particle size, size distribution, and zeta potential determination showed relatively stable dosage form.
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