PENGEMBANGAN FORMULA NANOEMULSI SPONTAN DENGAN PEMANFAATAN ARTIN-M DART BIJI ARTOCARPUS INTEGRIFOLIA SEBAGAI ADJUYAN VAKSIN TRANSDERMAL

• Main Author: Aliyandi, Aldy
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/64303
• Institution: Institut Teknologi Bandung

Vaccine is a preparation containing immunogenic compound with additional adjuvant to enhance immune response. Vaccine is commonly administered via parenteral route, however, it shows some disadvantageous. This research aimed to develop transdermal vaccine formulation consisted of nanoemulsion base, bovine serum albumin (BSA) as model immunogen protein, and artin-M as adjuvant. Artin-M is a lectin isolated from cempedak (Artocarpus integrifblia) seed. Nanoemulsion formulation was prepared using self-nanoemulsification method as the oil in water emulsion. Nanoemulsion was prepared using a mixture of virgin coconut oil (VCO), Tween 80, and PEG 400 as oil phase, surfactant, and co-surfactant, respectively. BSA and artin-M were incorporated into oil phase as solid dispersion form using PEG 20000. Evaluation of nanoemulsion consisted of BSA entrapment efficiency, hemagglutination activity of artin-M, and physicochemical stability test including the measurement of globule size, polydispersity index, viscosity, zeta potential, and pH. The optimum formula of nanoemulsion comprised of 3% VCO, 16% Tween 80, 8% PEG 400, 5 mg/g solid dispersion of BSA, and 50 ug/g solid dispersion of artin-M. The average of globule diameter was 42.7 ± 0.9 nm and polydispersity index was 0.321 ± 0.01. BSA entrapment efficiency in oil phase was 98.6 + 0.15%. Hemagglutination activity test showed that artin-M in the nanoemulsion had activity up to 3.12 ppm. The stability test showed that nanoemulsion was relatively stable during 24 days of storage.