#TITLE_ALTERNATIVE#

• Main Author: (NIM : 20504017), Mastura
• Format: Theses
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/6440
• Institution: Institut Teknologi Bandung

abstract: <br /> <br /> <br /> <br /> <br /> Mitochondria is an organelle of the eukaryote cell that functions as the energy generating system which produce adenosine triphosphate (ATP). The complete sequence of genome human DNA mitochondria (mtDNA) have been determined and recognized as the Cambridge Reference Sequence (CRS) nucleotide sequence, and become the standard reference in molecular genetics study especially related to human mtDNA polymorphism. Preview researchers used RFLP (Restriction Fragment Length Polymorphism) as one of the methods to determine the evolution relationship among ethnical groups. Research concerning human mtDNA polymorphism base on restriction enzyme have succeededly grouped the original population of America into haplogroup A, B, C, and D. The research consist of <br /> <br /> <br /> <br /> <br /> several and takes, namely determination of amount, complete nucleotide sequence mutation position and type of human mtDNA using a mito mutation analyzer (MMA) programe, and determination of the haplogroup by using 14 endonuclease restriction enzymes. The enzymes used are HaeIII, AluI, HincII, HhaI, HaeII, HpaI, RsaI, MboI, AvaII, BamHI, NIaIII, HinfI, TaqI, and AccI <br /> <br /> <br /> <br /> <br /> The analysis of 584 the nucleotide sequence of human mtDNA using the above software revealed the amount and the position of mutation and also the insersion or <br /> <br /> <br /> <br /> <br /> deletion. The highest amount of the mutation is 129 position with the lower is 12 position. The determination of haplogroup base on the mutation position of the <br /> <br /> <br /> <br /> <br /> complete nucleotide sequence showed variative haplogroups. Based on this analysis almost all the samples could be associated into several haplogroups and some sample could be long to more than one haplogroups. Majority of samples belong to one haplogroups, namely H and F haplogroups, and the rest of sample belong to two, <br /> <br /> <br /> <br /> <br /> three, and four haplogroups respectively. The higst degree of similarity between haplogroup in this research and those which based on RFLP are shown in individuals <br /> <br /> <br /> <br /> <br /> owning one haplogroup. It could be concluded due to excistence of individual owning more than one haplogroup or individual withoud any haplogroup, a new haplogroup based on nucleotide mutation showed be created.