CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT

CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT

Abstract : <br /> <br /> <br /> <br /> Industrial utilization of chitin on large scale has not been achieved efficiently because so far there is no enzyme available that hydrolyzes B-1,4 linkage between N-acetyl-glucosamine in crystalline chitin without pretreatment with...

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Main Author: Sari (NIM 107 03 007) , Meinila
Format: Final Project
Language:Indonesia
Online Access:https://digilib.itb.ac.id/gdl/view/6450
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Institution: Institut Teknologi Bandung
Language: Indonesia
id id-itb.:6450
spelling id-itb.:64502012-06-19T14:29:32ZCONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT Sari (NIM 107 03 007) , Meinila Indonesia Final Project INSTITUT TEKNOLOGI BANDUNG https://digilib.itb.ac.id/gdl/view/6450 Abstract : <br /> <br /> <br /> <br /> Industrial utilization of chitin on large scale has not been achieved efficiently because so far there is no enzyme available that hydrolyzes B-1,4 linkage between N-acetyl-glucosamine in crystalline chitin without pretreatment with hydrochloric acid. Therefore, acidic chitinase is required for that purpose Chromobacterium violaceum is bacterium isolated from Indralaya swamp area located in South Sumatera and is expected to produce acidic chitinase. C. violaceum was confirmed bt Gram staining and Polymerase Chain Reaction (PCR) 16s rDNA as well as to be chitinase producer using qualitative chitinase activity assay in Nutrient Agar medium containing colloid chitin. The nucleotides predicted to contain a fragment gene encoding the first 197 amino acids of chitinase has been cloned into pGEM-T in E. coli JM 109 based data obtained from migration analysis, PCR analysis, and cleavage analysis by restriction enzyme. <br /> text
institution Institut Teknologi Bandung
building Institut Teknologi Bandung Library
continent Asia
country Indonesia
Indonesia
content_provider Institut Teknologi Bandung
collection Digital ITB
language Indonesia
description Abstract : <br /> <br /> <br /> <br /> Industrial utilization of chitin on large scale has not been achieved efficiently because so far there is no enzyme available that hydrolyzes B-1,4 linkage between N-acetyl-glucosamine in crystalline chitin without pretreatment with hydrochloric acid. Therefore, acidic chitinase is required for that purpose Chromobacterium violaceum is bacterium isolated from Indralaya swamp area located in South Sumatera and is expected to produce acidic chitinase. C. violaceum was confirmed bt Gram staining and Polymerase Chain Reaction (PCR) 16s rDNA as well as to be chitinase producer using qualitative chitinase activity assay in Nutrient Agar medium containing colloid chitin. The nucleotides predicted to contain a fragment gene encoding the first 197 amino acids of chitinase has been cloned into pGEM-T in E. coli JM 109 based data obtained from migration analysis, PCR analysis, and cleavage analysis by restriction enzyme. <br />
format Final Project
author Sari (NIM 107 03 007) , Meinila
spellingShingle Sari (NIM 107 03 007) , Meinila
CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT
author_facet Sari (NIM 107 03 007) , Meinila
author_sort Sari (NIM 107 03 007) , Meinila
title CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT
title_short CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT
title_full CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT
title_fullStr CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT
title_full_unstemmed CONFIRMATION CHROMOBACTERIUM VIOLACEUM AS MICROORGANISM PRODUCE CHITINASE AND CLONING ITS GENE FRAGMENT
title_sort confirmation chromobacterium violaceum as microorganism produce chitinase and cloning its gene fragment
url https://digilib.itb.ac.id/gdl/view/6450
_version_ 1820663896176852992

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