#TITLE_ALTERNATIVE#
Abstract: <br /> <br /> <br /> <br /> Streptococcus pneumoniae clinical isolate was confirmed using microbiological, biochemical and molecular methods. The isolate showed proper characteristic of S.pneumoniae i.e. belongs to Gram positive, the presence of hemolysis, optoch...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/6452 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Abstract: <br />
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Streptococcus pneumoniae clinical isolate was confirmed using microbiological, biochemical and molecular methods. The isolate showed proper characteristic of S.pneumoniae i.e. belongs to Gram positive, the presence of hemolysis, optochin sensitive, but negative for sodium deoxycholate solubility test. The nucleotide sequences of 16S rDNA showed high identity to S. pneumoniae, S. mitis and S. oralis. Determination of serotype was done by amplify partial cpsA-cpsB fragment using primer cpsS1 and cpsA3 in several PCR conditions and compositions; however, PCR did not give any PCR product. Isolation of capsular polysaccharide was successfully done and a hexoamine homolog which is one of capsular polysaccharide component of S. pneumoniae. |
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