AKTIVITAS ANTIOKSIDAN, TOTAL FENOL, FLAVONOID, KAROTENOID DAM BERBAGAI EKSTRAK DAUN MENGKUDU (MORINDA CITRIFOLIA L.) DAN ISOLASI SENYAWA ANTIOKSIDAN

• Main Author: Fatmawati, Yeni
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/64757
• Institution: Institut Teknologi Bandung

Nowadays, there are increase of pollution such as air pollution, VaktI pollution, and u1Via\yolet radiation has been posn-i‘1, a tkeat.V)1\ux-i-YanVe. Some of the pollution are free radicals which lead to oxidative stress in body and resulting various illnesses as cancer, tumors, and other degenerative diseases. Therefore we need a compound that can neutralize and reduce the negative effect of free radical. Antioxidants are compounds that may prevent the formation of free radicals and may inhibit the oxidation reaction by converting free radicals and highly reactive molecules into a more stable compound. Based on several studies, noni leaf (Morin(la citrifolia L.) was known to have the effect of antioxidant. In addition, noni leaves also has been used by people as a medicine to treat various diseases. The aims of this research are to determine antioxidant capacity from various extracts of Morinda citrifolia leaves using DPPH assay, 1050 of DPPH scavenging activity, phenolic total, flavonoid total, carotenoid total of each extracts, analyze correlation of phenolic total,flavonoid total, carotenoid total to DPPH scavenging activity and isolation of antioxidant compound from noni leaves. Crude drug of noni leaves was extracted using reflux aparatus with three diferent polarity solvents n-hexane, ethyl acetate, and ethanol. For each extract were conducted monitoring extract by TLC (thin layer chromatography), antioxidant activity test by using DPPH (2,2-dipheni1-1-picrillndrazyl) radical scavenging method and IC50 of DPPH scavenging capacity, total phenolic, total flavonoid and total carotenoid content. Ethyl acetate extract was fractioned using vacuum liquid chromatography. Purification was done using column chromatography. Antioxidant compound was characterized using spesific spray reagent, UV-Vis spectrophotometry and two dimensional paper chromatography. Purity test of subfraction was done by TLC single development with three different mobile phases. Crude drug of noni leaves contained flavonoids, quinones, phenolic compounds, and steroids/triterpenoids groups. Ethanolic extract of noni leaves (with density of extract 1% 0,82 g/mL) showed the highest DPPH scavenging activity (58,46%), IC50 15,25 tg/mL, phenolic total 4,09 g GAE/100 g, flavonoid total 3,94 g QE/100 g, carotenoid total 1,39 g BET/100 g. DPPH scavenging capacity of ethanolic extract significally different with ethyl acetate extract and n-hexane extract (p<0,05). The antioxidant compound X was isolated from ethyl acetate extract. The antioxidant activity of ethanol extract of noni leaves was the highest and significantly different with ethyl acetate extract and n- hexane extract. Content of flavonoid total, phenolic total, and carotenoid total in n-hexane, ethyl acetate, and ethanol of noni leaves had no significant correlation with DPPH scavenging activity. Antioxidant compound X that was isolated from ethyl acetate extract of noni leaves was predicted to be flavonol aglicone, that had free OH in C3.