DISOLUSI ANDROGRAFOLIDA DALAM SIMPLISIA DAN EKSTRAK HERBA SAMBILOTO (ANDROGRAPHIS PANICULATA NESS.)

• Main Author: Nakatama Parapat, Niko
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/64760
• Institution: Institut Teknologi Bandung

Andmgraphis paniculata Ness. (sambiloto) is one of the medicinal plants that grow well in Indonesia. Sambiloto has been widely used as a medicinal plant to treat various diseases. One of the pharmacologically active compounds in Andrographis. paniculata Ness. is andrographolide. The objectives of this research was to isolate andrographolide marker and to determine the amount of dissolved andrographolide in samples in the dissolution medium. Andrographolide marker was isolated from crude drug using Soxhlet apparatus with acetone. Acetone extract was monitored by thin layer chromatography (TLC) with eluen chloroform - ethanol (8.5 : 1.5) and spray reagent vanilin sulfuric acid. Characterization of isolates using TLC with spray reagent vanillin sulfuric acid and determination of the maximum wavelength (kn,ax) isolates using UV-Vis Spectrophotometry. Samples for dissolution tests were crude drug, dried ethanol extract, freeze dried water extract, and boiled water extract. Dissolution test apparatus that was used in this reaserch was paddle apparatus of Hanson SR8-PLUS. Dissolution medium was used are 0.1 N HC1 pH 1.2 and pH 6.8 phosphate buffer with 900 mL volume. The speed of paddle is 50 rpm. Time testing was conducted for 1 hour with a sampling point at minute 10, 20, 40, and 60. Determination of andrographolide in the samples was done by HPLC method using a mobile phase of methanol - water (gradien elution). Calibration curve for the determination of andrographolide in samples was made using the andrographolide marker solution. Crude drug, water extract, and ethanol extract contains flavonoids, phenols, steroids-triterpenoids, and terpenoids. Characterization of isolates by UV-Vis spectrophotometry showed the maximum X isolates at 223 nm. Percent andrographolide dissolved in 0.1 N HC1 pH 1.2 in each sample was (a) crude drug 9.46; 12.70; 23.01; 31.80 (b) boiled water extract 4.10; 4.80; 6.72; 6.72 (c) dried water extract 2.20; 4.04; 4.41; 4.58 (d) dried ethanol extract 1.89; 2.19; 2.52; 4.03. Percent andrographolide dissolved in phosphate buffer pH 6.8 in each sample was (a) crude drug 4.54; 6.39; 8.66; 9.85 (b) boiling water extract 3.82; 4.22; 3.81; 5.05 (c) dried water extract 1.86; 2.06; 2.33; 2.47 (d) dried ethanol extract : can not be determined because the dissolved of andrographolide was too small. Water soluble extract and ethanol soluble extract from crude drug respectively are 23% and 10%. Amount of dissolved andrographolide in crude drug is higher than in boiled water extract, freeze dried water extract, and dried ethanol extract (in medium HCl pH 1.2 and phosphate buffer p1-1 6.8). Percent andrographolide dissolved in HCl 0.1 N pH 1.2 is better than in buffer phosphate pH 6.8.