AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN, BATANG, DAN AKAR PASAK BUMI (EURYCOMA LONGIFOLIA JACK) DENGAN METODE DPPH DAN CUPRAC

• Main Author: Hakisa Rendra, Aristo
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/65594
• Institution: Institut Teknologi Bandung

Free radicals are reactive chemical species produced inside the body or obtained from the environment which can cause diseases, such as cancer. Antioxidants are compounds that can scavenge free radicals. One of the potential plants as a natural antioxidant is longjack. The objectives of this research were to determine the antioxidant activity of leaves, stems, and roots of longjack using DPPH and CUPRAC methods, total phenolic content (TPC), total flavonoid content (TFC), the correlation between TPC and TFC with antioxidant activity, the correlation between the two methods, and the identification and determination of marker content in the highest-yield extract. Extraction was performed by reflux using n-hexane, ethyl acetate, and ethanol. Antioxidant activity using DPPH and CUPRAC, TPC, and TFC was determined by UV-visible spectrophotometry. Identification and quantification of marker in the highest-yield extract were determined by high performance liquid chromatography. DPPH antioxidant activity of leaves, stems, and roots extract of longjack in the range of 2.03-39.08 mg AAE/g, while CUPRAC in the range of 10.45-60.12 mg AAE/g. The highest TPC was given by ethyl acetate stems extract (6.81 ± 0.21 g GAE/100 g), while the highest TFC was given by nhexane roots extract (4.70 ± 0.28 g QE/100 g). The TPC and TFC of longjack extracts had significantly positive correlation with their antioxidant activities by DPPH and CUPRAC methods, except ethyl acetate stems extract. DPPH and CUPRAC had positive and significant correlation. In general, phenolic and flavonoid compounds gave great contribution on antioxidant activity of longjack by DPPH and CUPRAC methods. The two methods showed linear results in determining the antioxidant activities of longjack extracts. The marker compound identified in ethanol leaves extract was luteolin-7-Oglucoside with a concentration of 0.103%.