BIOMIMETIC HYDROGENATION OF GLUCOSE TO HEXITOL

• Main Author: Sabrina
• Format: Final Project
• Language: Indonesia
• Subjects: Teknik (Rekayasa, enjinering dan kegiatan berkaitan)
• Online Access: https://digilib.itb.ac.id/gdl/view/65711
• Institution: Institut Teknologi Bandung

Aldose reductase enzyme is known as an enzyme that catalyzes the hydrogenation conversion of monosaccharides to sugar alcohol at room temperature with NADPH and H+ as the hydrogen donors. Further studies suggest a higher possibility of hydrogen sources come from HCOOH (formic acid) and other catalyst candidates, such as (NixFe1-x)MoS4, (NipFe1-p)(CrqFe1-q)2S4 or Fe3S4, (Ni½Zn½)(OOCH)2.2H2O, and Ni(MIII)2O(OOCH)6.3H2O, with M ? Cr and Fe, which have the potentials to act as a catalyst by imitating how enzymes work (biomimetic). The objective of this research is to obtain a proof towards hypotesis regarding the effectiveness of catalyst candidates and to gain knowledge about how the operating conditions affect the activity of the best catalyst chosen from the experiment. At the beginning, the catalyst is put into a glucose solution, that has been added formic acid and triethylamine, with pH 4,5 – 6,5, followed with mixing the solution at reflux atmospheric condition (95 ?) for 6 hours. The top of condenser reflux is connected to a gas-washing bottle for the purpose of capturing CO2 as the by-product of monosaccharides reduction. Analyses will be conducted toward the mixed solution, including using modification of Lane-Eynon method to determine the conversion degree of glucose and paper chromatography to prove the presence of sorbitol. Experimental result proves that catalyst candidates, (NixFe1-x)MoS4 catalyst with x = ½ dan ¾, Fe3S4 catalyst, (NipFe1-p)(CrqFe1-q)2S4 catalyst with p = ½ and q = ¼, (Ni½Zn½)(OOCH)2.2H2O catalyst, and Ni(MIII)2O(OOCH)6.3H2O catalyst with M ? Cr and Fe have great catalytic activities toward biomimetic hydrogenation of glucose to hexitol.