AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN, KULIT BATANG, DAN RANTING KAYU MANIS (CINNAMOMUM BURMANNII (NEES & T. NEES) BLUME) DENGAN METODE DPPH DAN CUPRAC
Free radical can have a toxic role. Antioxidant is a molecule that can neutralize free radicals. Cinnamon was known to possess antioxidant activity. This research aims to determine antioxidant activities of leaves, barks, and branches of cinnamon with DPPH and CUPRAC methods; total phenol and fla...
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| Format: | Final Project |
| Language: | Indonesia |
| Online Access: | https://digilib.itb.ac.id/gdl/view/65787 |
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| Institution: | Institut Teknologi Bandung |
| Language: | Indonesia |
| Summary: | Free radical can have a toxic role. Antioxidant is a molecule that can neutralize free radicals.
Cinnamon was known to possess antioxidant activity. This research aims to determine antioxidant
activities of leaves, barks, and branches of cinnamon with DPPH and CUPRAC methods; total phenol
and flavonoid; analyze correlation of total phenol and flavonoid with antioxidant activities by DPPH
and CUPRAC; correlation between DPPH and CUPRAC methods, identify and determine marker
content in extract. Extraction was done by reflux alternately using n-hexane, ethyl acetate and
ethanol solvents. DPPH and CUPRAC assay together with total phenol and flavonoid content
measurement was done using UV-visible spectrophotometry and followed by Pearson correlation.
Identification and determination of marker content was conducted using HPLC. Antioxidant
activities of cinnamon extracts were in the range of 5.369-775.649 mg AAE/g (DPPH) and 10.488-
561.193 mg AAE/g (CUPRAC). Highest antioxidant activity with two methods found at branches
ethanol extract. Ethanol barks extract had the highest phenol content (22.423 ± 1.084 g GAE/100
g), while ethyl acetate leaves extract has the highest flavonoid content (6.272 ± 0.301 g QE/100 g).
Total phenol and flavonoid had a significant positive correlation with antioxidant activities by DPPH
and CUPRAC. Correlation of antioxidant activities between DPPH and CUPRAC methods of all
cinnamon extracts were significantly positive, except for the branches n-hexane extract. Phenolic
and flavonoids compounds group had great contribution to antioxidant activities of cinnamon
extracts by DPPH and CUPRAC methods. DPPH and CUPRAC methods gave linear results in
antioxidant activities of cinnamon extracts. The identified marker compound of ethanol barks
extract of cinnamon was eugenol, with concentration of 0.000569%.
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