HEAVY METAL EXPOSURE ANALYSIS THROUGH DERMAL PATHWAY AND THEIR POTENTIAL EFFECT ON DNA DAMAGE IN BATIK WORKERS (CASE STUDY: HOUSEHOLD-SCALE BATIK INDUSTRY AT LENDAH DISTRICT, KULON PROGO, YOGYAKARTA)

• Main Author: Oclandhi, Buggie
• Format: Theses
• Language: Indonesia
• Subjects: Teknik saniter dan perkotaan; teknik perlindungan lingkungan
• Online Access: https://digilib.itb.ac.id/gdl/view/66199
• Institution: Institut Teknologi Bandung

The process of making batik using synthetic dyes containing heavy metals. Batik workers have the potential to be exposed to heavy metals through the dermal exposure route which can have a negative impact on workers' health. The purpose of this study was to determine the level of heavy metal exposure through the dermal exposure route and to determine the relationship between the level of heavy metal exposure in the skin and the level of DNA damage. This study was conducted with a cross-sectional epidemiological approach. Dermal exposure sampling was carried out using an MCE filter that was attached to an exposed body part for 4 hours. The filter was tested using XRF with heavy metal parameters Cr, Mn, Fe, Ni, Cu and Zn. Blood samples were taken as much as 2 ml. DNA damage examination was carried out using the Comet Assay method. From the test results of the dermal patch samples that were attached to each respondent, it was found that the heavy metal content was found in both the worker and control groups. The average concentration of heavy metals in the patch workers group was higher than the control group. The results of the HI value for dermal heavy metal compounds by batik workers have not yet exceeded 1, which means that exposure to heavy metals through the dermal route has the potential to cause minimum effects that are harmful to workers. Based on the results of the Comet Assay, the 3 main parameters Tail Length, Tail DNA% and Tail Moment in the exposed group had a higher average value than the control group. The dyeing job parameter has an OR value of 4.24 (OR>1) which indicates that the dyeing worker has a 4.24 times higher probability of influencing the increase in Tail DNA% compared to the canting and tasting jobs. The parameter of working period of more than 5 years has an OR value of 2.04 (OR>1) which indicates that workers with a working period of more than 5 years have a 2.04 times higher probability of influencing the increase in Tail DNA% compared to a period of work of less than 5 years. year.