KAJIAN EVOLUSI MANGANESE SUPEROXIDE DISMUTASE (MNSOD) BAKTERI BERDASARKAN URUTAN ASAM AMINO

• Main Author: Audina Haryantho, Feonie
• Format: Final Project
• Language: Indonesia
• Online Access: https://digilib.itb.ac.id/gdl/view/66205
• Institution: Institut Teknologi Bandung

Manganese superoxide dismutase (MnSOD) is a ubiquitous metalloenzyme that presents among microorganisms and plays an important role in eliminating reactive oxygen species (ROS) in body tissue. However, the amount of MnSOD is decreasing along with aging. Exogenous MnSOD can be added to maintain MnSOD level in the body. MnSOD from Staphylococcus equorum (MnSODSeq) has been developed in the Pharmaceutical Biotechnology Laboratory of the School of Pharmacy, ITB. The aim of the study was to examine the evolution of MnSODSeq and asses its relationship to other MnSOD from different organism and to determine the evolution marker based on phylogenetic tree analysis. A phylogenetic tree based on the amino acid sequence was generated using MEGA11 software. The result showed that MnSOD from pathogenic bacteria is grouped differently from the nonpathogenic ones. An analysis was conducted to determine the effect of different amino acids among the MnSOD sequences. The result showed that different amino acid affects the activity, structure, and stability of MnSOD. However, there was no specific pattern regarding the effect for each group of MnSOD in the phylogenetic tree. Every difference found was particular for each species. MnSOD from pathogenic bacteria have three amino acids that are only conserved in their group; they are Asp36, Lys59, and Asp105 (numbering based on Escherichia coli MnSOD). However, the electrostatic potential surface analysis showed that these amino acids did not form any specific pattern and thus the conservation is unlikely related to the pathogenicity of bacteria.